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Fermentation Lab. Fermentation Equation. C 6 H 12 O 6 2C 2 H 5 OH + 2CO 2 + 2 ATP Glucose ethanol + carbon dioxide + 2 ATP . Anaerobic vs. Aerobic. Anaerobic = without oxygen C 6 H 12 O 6 2C 2 H 5 OH +2CO 2 +2 ATP
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Fermentation Equation • C6H12O6 2C2H5OH + 2CO2 + 2 ATP • Glucose ethanol + carbon dioxide + 2 ATP
Anaerobic vs. Aerobic • Anaerobic = without oxygen • C6H12O6 2C2H5OH +2CO2 +2 ATP Glucose ethanol + carbon dioxide +2 ATP • Aerobic = with oxygen
Alcohol Fermentation • Must be airtight (or it will go into an aerobic respiration) • By yeast and some kinds of bacteria
We’re Using Yeast • Saccharomyces Cerevisae. • Note Budding
CO2 Produces Holes in Bread • Yeast product of carbon dioxide makes holes as it expands in the bread
Water Bath: A beaker inside of a beaker • This keeps the water temperature even
Ideal Growing Conditions 1. moisture 2. a temperature between 10 and 50ºC but ideally 30-37ºC 3. a food source such as sugar or flour • WATCH THE THERMOMETER!!!!! • Keep it in the beaker.
Gas Collection Tubes • Take readings from tube • Holes already in lid • Invert in warm water to act as waste container for ethanol
Running a little slow… • Sheldon will give your solution a boost… • (yeast loading) Dry Dormant Stage