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Details 11.5.04

Details 11.5.04. confocal stacks of double staining were taken with 60x obj. 2.5xzoom, 2x int and fs Green channel = ATP synthase (PMT 2100) Red channel = IGFR (PMT 1900) or C9 (PMT 2400) NO image processing has been applied save minor level adjustments. IGFR/ATPsynthase stack 1.

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Details 11.5.04

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  1. Details 11.5.04 confocal stacks of double staining were taken with 60x obj. 2.5xzoom, 2x int and fs Green channel = ATP synthase (PMT 2100) Red channel = IGFR (PMT 1900) or C9 (PMT 2400) NO image processing has been applied save minor level adjustments.

  2. IGFR/ATPsynthase stack 1

  3. IGFR/ATPsynthase stack 2 Untransfected, normal cell

  4. IGFR/ATPsynthase stack 3

  5. C9/ATP synthase stack1

  6. C9/ATP synthase stack2

  7. C9/ATP synthase stack 3

  8. C3 imaging details • Confocal single plane images • 20x obj, 1x zoom, 2x int, 2xfs • Blue = nuclei (PMT = 1430) • Red = C3 staining (PMT = 2500)

  9. C3 in C9 transfection

  10. C3 in pcna control

  11. C3 in IGFR transfection

  12. C3 in NK1R transfection

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