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CaMV35S/ GT

Trzy typy R, typ II (Mg) nacina w miejscu rozpoznania, III (ATP, Mg, SAM), około 1000bp od tego miejsca, II (ATP, Mg) 24-26 bp HaeIII 4 nt, tępe, SmaI 6 nt, tępe, większość 5’-overhang, PstI 3’-overhang HpaII/MspI C’CGG MboI/Sau3AI G’ATC. CC. GC. Petunia hybrida DFR cDNA. CaMV35S/ GT.

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CaMV35S/ GT

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  1. Trzy typy R, typ II (Mg) nacina w miejscu rozpoznania, III (ATP, Mg, SAM), około 1000bp od tego miejsca, II (ATP, Mg) 24-26 bp HaeIII 4 nt, tępe, SmaI 6 nt, tępe, większość 5’-overhang, PstI 3’-overhang HpaII/MspI C’CGG MboI/Sau3AI G’ATC

  2. CC GC

  3. Petunia hybrida DFR cDNA CaMV35S/ GT OCS CTCCGGTG Me Msp I Hpa II 5’ ... C C G G ... 3’ 3’ ... G G C C ... 5’ 5’ ... C C G G ... 3’ 3’ ... G G C C ... 5’ Me * Me * CCGG CCGG T 21 25 30 40 49 * *

  4. pGT-DFR Desi 6 8 10 29 30 Ekspresja transgenu jest regulowana przez metylację

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