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140 micro

بسم الله الرحمن الرحيم. 140 micro. Lab 2 : Culture Media. Microorganisms need food to grow. Primary ingredients required by all living organisms include: a carbon source, water, minerals, and a nitrogen source. These nutrients together make a media.

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140 micro

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  1. بسم الله الرحمن الرحيم 140 micro Lab 2: Culture Media

  2. Microorganisms need food to grow • Primary ingredients required by all living organisms include: a carbon source, water, minerals, and a nitrogen source. • These nutrients together make a media. • Different microbes need different amounts of these nutrients.

  3. Culture media may be found in one of three states: • liquid (called broth). • semi-solid. • solid.

  4. Aim To prepare solid and Liquid media

  5. Requirement • Deffrent media : Nutreint broth - Nutrient agar - PDA/malt extract

  6. Requirement 2- Balance 3- Distil water 5- Test tubes 6- Petri plates 7- Flaskes 7- Burners 8- Autoclave

  7. Procedure 1- Weigh 2- Dissolve 3- Sterilize 4- cool 5- refrigerate till use

  8. 1- Weigh

  9. 2- Dissolve

  10. 3- Sterilize

  11. 4- cool 5- refrigerate till use

  12. Who to make …….?

  13. Pouring of Solid media: - Petri plates • Remove the lid slightly • Pour the media near bunsen burner • Invert the plate • Write date and time on the sides of plates

  14. - Making of Slants: • After boiling, pour media in test tubes • Autoclave • Place in slant position till the media solidfys.

  15. - Agar deep tube : • After boiling, pour media in test tubes • Autoclave • Place in vertical position till the media solidfys.

  16. Slant agar - Deep agar

  17. -Making of test tubes with broth media : • Place the testube near a burner and remove the cap • Pour the media in the tube and close the cap at once • Place the tube in upright position in the testube stand

  18. Thank you ….

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