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Capturing Circulating Tumor cells using Nanotechnology

Kyle Rafferty Biomedical Engineering. Capturing Circulating Tumor cells using Nanotechnology . Overview. What are CTC’s? Why is detection important? Older methods of detection Immunomagnetic beads Flat silicon substrates New Advancement Silicon nanopillar substrate Sources. CTC’s.

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Capturing Circulating Tumor cells using Nanotechnology

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  1. Kyle Rafferty Biomedical Engineering Capturing CirculatingTumor cells using Nanotechnology

  2. Overview • What are CTC’s? • Why is detection important? • Older methods of detection • Immunomagnetic beads • Flat silicon substrates • New Advancement • Silicon nanopillar substrate • Sources

  3. CTC’s • Circulating Tumor Cells • Metastasis • Deadly • Cancer becoming biggest killer in world (WHO) • Most common death from metastasis • Helpful to know amount in bloodstream • Examining cancer metastasis • Predicting patient prognosis • Monitoring cancer

  4. Previous Methods • Immunomagnetic Beads • “Utilizes capture-agent-coated magnetic beads to immunologically recognize CTCs in the blood, followed by magnetic isolation. However, these bead-based approaches are limited by their low CTC-capture yield and purity.” • Use ferrofluids(microscopic iron particles)/antibodies and magnets to extract • CellSearch • Flat Silicon Substrate • 1x2 cm silicon chip • Coated with streptavidin and anti-EpCAM (epithelial cell adhesion molecule antibody)

  5. Silicon Nanopillar Substrate • Build much like flat silicon • 1x2 cm silicon ship • Built by lithographic tools used for computer chips • Difference: Nanopillars • Diameter~100-200nm; Length~20um • Also coated with streptavidin and anti-EpCAM (epithelial cell adhesion molecule antibody)

  6. SiNP Substrate cont. • Researchers found maximum cell-capture numbers were at 45 min. incubation time • As compared to 3-4 hrs for previous method • This is more efficient and therefore would probably cut costs PBS: Phosphate buffered Saline. Very common biological solution.

  7. Diagram: how SiNP works

  8. Diagram: Si vs. SiNP

  9. Future Use • Researchers hope to achieve 84-91% detection • Will allow faster detection of cancer’s • Allow Doctor’s to customize treatment plan • Increase early detection and survival rates

  10. Sources • Wang, Shutao. “Three-Dimensional Nanostructured Substrates toward Efficient Capture of Circulating Tumor Cells.” Angewandte Chemie International Edition 48 (2009): 8970-8973. 26 Jan. 2010. http://www3.interscience.wiley.com/cgi-bin/fulltext/122658940/PDFSTART (impact factor: 10.879) • (2) Berger, Michael. “Nanotechnology trap captures cancer cells in blood.” Nanowerk, Nov. 30 2009. 26 Jan. 2010. http://www.nanowerk.com/spotlight/spotid=13743.php • Wikipedia contributors. "Metastasis." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 23 Jan. 2010. Web. 26 Jan. 2010. • Stobbe, Mike. “Cancer top killer in world by 2010.” The Seattle Times, 10 Dec. 2008. 26 Jan. 2010. http://seattletimes.nwsource.com/html/health/2008489006_cancer10.html

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