1 / 13

Isolation of Rare Circulating Tumor Cells

Isolation of Rare Circulating Tumor Cells. Sunitha Nagrath et al. Presentation by: Keara Darragh , Matthew Faw , and Will Plautz. http://www.nibib.nih.gov/news-events/newsroom/circulating-tumor-cells-captured-last. Overview of Metastasis and CTCs. Circulating tumor cells (CTCs)

vesta
Download Presentation

Isolation of Rare Circulating Tumor Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Isolation of Rare Circulating Tumor Cells Sunitha Nagrath et al. • Presentation by: • KearaDarragh, Matthew Faw, and Will Plautz http://www.nibib.nih.gov/news-events/newsroom/circulating-tumor-cells-captured-last

  2. Overview of Metastasis and CTCs • Circulating tumor cells (CTCs) • Likely the origin of intractable metastatic disease • 1 CTC : 10^9 haematologic cells in the blood of cancer patients

  3. CTC chip better than existing methods • Immunomagnetic-bead • purification Circulating Tumor Cell (CTC) Chip • First microfluidic device that can successfully isolate viable cells frommilliliter volumes of whole blood • High sensitivity, selectivity, and yield • Single step • Gross prognostic tool (high/low risk) • Isolates fewerCTCs with very low purity (<1%)and yield • Multiple bulk semi-automatedpreparatory steps

  4. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  5. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  6. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  7. How Does the Chip Capture Circulating Tumor Cells? • 25 mm x 66 mm silicon chip • 78,000 microposts • epCAM antibodies

  8. CTC Isolating Microfluidic Device 25mm

  9. Staining to Assess Purity • Stains are used in order to identify malignant cells from healthy cells. • Malignant cells look different • Large cellular size • Relatively large nuclei • Visible nucleoli

  10. Results and Applications • Successfully identified CTCs in 115/116 samples • Approximately 50% purity • CTCs isolated (per ml): • NSCLC: 5-1,281 • Breast: 5-174 • Monitor cancer treatment response

  11. Results and Applications • Successfully identified CTCs in 115/116 samples • Approximately 50% purity • CTCs isolated (per ml): • NSCLC: 5-1,281 • Breast: 5-174 • Monitor cancer treatment response

  12. Conclusion • First microfluidic device that can successfully process milliliter volumes of whole blood • High sensitivity, selectivity, and yield • Single step • Isolation of viable cells • Allows for real time monitoring of cancer progression in a non-invasive manner

More Related