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Expansion of tandem gene clusters

Expansion of tandem gene clusters. Gene duplication by unequal crossing-over. Divergence of coding and regulatory sequences. Evolution of HOX clusters. The homeobox is a highly conserved DNA-binding domain. HOX4 homeodomain. Drosophila. Amphioxus. Mouse. Human. Chick. Frog. Fugu.

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Expansion of tandem gene clusters

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  1. Expansion of tandem gene clusters Gene duplication by unequal crossing-over Divergence of coding and regulatory sequences

  2. Evolution of HOX clusters

  3. The homeobox is a highly conserved DNA-binding domain HOX4 homeodomain Drosophila Amphioxus Mouse Human Chick Frog Fugu Zebrafish

  4. Evolution of HOX clusters in vertebrates - Vertebrates have multiple HOX clusters - Paralogous HOX genes may have partly redundant functions Mouse - Some genes and clusters become specialized for distinct functions Fugu - Different lineages lose some genes and acquire new functions for others Zebrafish

  5. Using gene replacement to test HOX gene function

  6. Using gene replacement to test HOX gene function

  7. Functionally equivalent HOX paralogs

  8. HOX gene labial required for tritocerebral commissure

  9. Genetic rescue strategy - lab-Gal4 lab ; lab-Gal4 / UAS - lab red = HRP; green = Lab

  10. Other HOX genes can partially substitute for lab

  11. Other HOX genes can partially substitute for lab

  12. Distinct roles of Ubx and abd-A in the midgut dpp expression in the fly midgut is activated by Ubx, but repressed by abd-A Activation and repression are mediated by the same binding sites within a dpp enhancer

  13. Onychophoran Ubx causes homeotic transformations in Drosophila

  14. Onychophoran Ubx can regulate some Drosophila target genes dSRF dpp

  15. Onychophoran Ubx cannot fully substitute for Drosophila Ubx

  16. Chimeric Ubx proteins

  17. Mapping the functional specificity of Onychophoran Ubx

  18. Functional specificity of Ubx maps outside the homeodomain

  19. Conservation and change in Ubx proteins

  20. Mapping the functional specificity of Ubx proteins

  21. Functional comparison of Drosophila and Artemia Ubx

  22. Evolution of a new repression domain

  23. Non-HOX functions of derived HOX genes

  24. Maternal gradient of bicoid establishes Anterior-Posterior axis in the Drosophila embryo

  25. bicoid gradient formation in Drosophila

  26. zen functions in Dorso-Ventral patterning sna sog zen

  27. zen is a derived HOX gene

  28. HOX-like expression of zen homologue in Cupiennius

  29. Evolution of zen and bcd from an ancestral HOX gene

  30. Genetic control of segmentation in Drosophila

  31. ftz deletion does not affect segmentation in Tribolium Antennae

  32. HOX-like expression of the Chelicerate ftz homologue Archegozetes longisetosus

  33. Effects of ectopic ftz expression in Drosophila Dm 36% 4% Sg en expression

  34. A homeodomain-deficient ftz allele

  35. ftz expression in Schistocerca

  36. Homeotic antenna to leg transformations Dm-ftz Tc-ftz Tc-ftz Dll-Gal4 Sg-ftz

  37. Tribolium ftz retains homeotic potential wt Dm-ftz Tc-ftz Dm-Scr wt Dm-ftz Tc-ftz Dm-Antp

  38. Evolution of ftz function

  39. Comparison of ftz proteins between Drosophila and Schistocerca

  40. Expression and function of ftz-F1 ftz-F1 ftz ftz-F1 mutant en

  41. ftz-F1 encodes a nuclear hormone receptor

  42. Physical interaction between ftz and ftz-F1

  43. Transcriptional activation by ftz / ftz-F1 complex In yeast In Drosophila embryos

  44. Novel functions of divergent HOX genes

  45. Loss and gain of protein-protein interaction motifs

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