1 / 63

Vaccine War

Vaccine War. “Undergraduate Research Opportunities” will be held Today, Thursday, Jan 13, at 3:30 pm in SL120. http://www.pbs.org/wgbh/pages/frontline/vaccines/. SEMINARS!!!!. Prof. Gregory Weiss , Department of Chemistry, University of California, Irvine .

tamarr
Download Presentation

Vaccine War

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Vaccine War “Undergraduate Research Opportunities” will be held Today, Thursday, Jan 13, at 3:30 pm in SL120 http://www.pbs.org/wgbh/pages/frontline/vaccines/

  2. SEMINARS!!!! Prof. Gregory Weiss, Department of Chemistry, University of California, Irvine The Weiss Laboratory pursues both chemical and biological aspects of chemical biology. Using chemistry to advance a molecular understanding of biology, the lab dissects key events in biology with exceptionally diverse combinatorial libraries as atomic-scale scalpels. Our libraries, collections of different molecules, include virus-displayed proteins and chemically or enzymatically synthesized small molecules. Libraries displayed on the surfaces of viruses also offer essentially universal molecular recognition for chemical sensors, illustrating how biology can advance chemistry.

  3. Phosphofructokinase (PFK) Works exactly like HK. Inhibited by hi [ATP] or citrate Activated by [AMP] even in the presence of hi [ATP].

  4. Mechanism animation

  5. Figure 17-8 Mechanism for base-catalyzed aldol cleavage. Page 589 Transition state analogs like 2-phosphoglycolate inhibit the enzyme

  6. Figure 17-9 Enzymatic mechanism of Class I aldolase. Page 590

  7. Enzyme-Substrate Complex trapped by reduction of DHAP with NaBH4 followed by hydrolysis

  8. Page 557 Figure 16-10 Mechanism of aldose–ketose isomerization.

  9. Figure 17-10 Proposed enzymatic mechanism of the TPI reaction: General Acid Catalysis. pKs = 6.5 and 9.5 Like PGI But pK1 is for GLU! Normal pk? 4.1 GluAsp  activity by 1000! Reaction rate is diffusion limited!!

  10. End of Glycolysis Collection Phase • Net result so far? • ATP • NAD+ • Carbon

  11. GAP DH Start of energy producing phase of glycolysis: Production of the first hi energy molecule.

  12. Figure 17-13a Some reactions employed in elucidating the enzymatic mechanism of GAPDH. (a) The reaction of iodoacetate with an active site Cys residue. (b) Quantitative tritium transfer from substrate to NAD+. Page 596 32Pi also incorporated

  13. Animated mechanism

  14. Figure 17-14 Enzymatic mechanism of glyceraldehyde-3 phosphate dehydrogenase. Go’ = +6.7 kJ! Page 596

  15. Figure 17-15 Space-filling model of yeast phosphoglycerate kinase showing its deeply clefted bilobal structure. Page 597

  16. Figure 17-16 Mechanism of the PGK reaction. Go’ = -12.1 kJ Go’ = -49.4 kJ! Page 597

  17. Phosphoglucomutase--PGM Animated mechanism Mutases move functional groups: 3PG2PG

  18. Figure 17-19 The pathway for the synthesis and degradation of 2,3-BPG in erythrocytes is a detour from the glycolytic pathway. Page 600

  19. Figure 17-20 The oxygen-saturation curves of hemoglobin (red) in normal erythrocytes and those from patients with hexokinase (green) and pyruvate kinase deficiencies (purple). BPG  BPG Page 600

  20. Figure 17-18 Proposed reaction mechanism for phospho-glycerate mutase. Phosphorylated active site Bisphospho- intermediate. Page 599

  21. Figure 17-22 Mechanism of the reaction catalyzed by pyruvate kinase.

  22. Let's sing!! http://www.csulb.edu/~cohlberg/songbook.html

  23. Lyrics http://books.google.com/books?id=oq9ENyL_d9YC&lpg=PP1&pg=PA1#v=onepage&q&f=false

  24. Figure 17-21 Proposed reaction mechanism of enolase. F- binds Pi + Mg+2 Potent inhibitor Page 601

  25. Figure 17-23 The active site region of porcine H4 LDH in complex with S-lac-NAD+, a covalent adduct of lactate and NAD+. Page 603

  26. Figure 17-24 Reaction mechanism of lactate dehydrogenase. Page 603

More Related