Gonorrhea and Chlamydia

1. Gonorrhea and Chlamydia David H. Martin, MD LSU Health Sciences Center Section of Infectious Diseases

2. Disclosure • I have no financial interests or other relationship with manufacturers of commercial products, suppliers of commercial services, or commercial supporters. My presentation will not include any discussion of the unlabeled use of a product or a product under investigational use.

3. An STD About to Happen!

4. Cell Monolayer Infected with Chlamydia and Stained with Chlamydia Specific Fluorescent Antibodies Cell cytoplasma counter stained red Chlamydial inclusions stained apple green

6. Electron Micrograph of a Chlamydia Infected Endocervix Columnar epithelial cells Chlamydial inclusion Microabscess

8. Gonococcal Urethritis

9. Gram Negative Intracellular Diplococci (GNID)

10. Nongonococcal Urethritis

11. Inflammation Without GNID

12. Etiology of Nongonococcal Urethritis - 2002 Chlamydia trachomatis 20-40% Ureaplasma urealyticum 20-40% ? Mycoplasma genitalium 20-25% Trichomonas vaginalis 1-5% Herpes simplex virus <1%

13. Epidydimitis Scrotalerythema Discharge

15. Gonococcal Endocervicitis

16. Chlamydial Endocervicitis

18. Etiology of PID

19. Diagnosis of PID History • Lower abdominal pain of less than 15 days duration • Constant pain • Onset within 7 days of menstruation • Fever and/or chills • Sexual history

20. Diagnosis of PID Signs • Bilateral adnexal tenderness • Cervical motion pain • Adnexal mass • Endocervical discharge • IUD

22. Maternal Chlamydial Infection 65% of infants are infected 5% of infants develop pneumonia 30 – 50% of infants develop conjunctivitis

23. Proportion of Chlamydial and Gonococcal Infections that are Asymptomatic

24. Epidemiology

25. U.S. Gonorrhea Rates: 1970–2000

26. Chlamydia — Trends in positivity among 15-44 year old women tested in family planning clinics, 1988–2000

28. Diagnosis

29. Gram Negative Intracellular Diplococci (GNID)

30. One Step Methylene Blue Stain

31. Performance of Culture for N. gonorrhoeae and C. trachomatis *Assuming no mistakes are made in specimen labeling and laboratory procedures.

32. Cell Monolayer Infected with Chlamydia and Stained with Chlamydia Specific Fluorescent Antibodies Cell cytoplasma counter stained red Chlamydial inclusions stained apple green

34. Performance of the Pace 2 Gonococcal DNA Hybridization Assay in Women

38. A Systematic Review of NAAT Sensitivity Using Urine, Cervical and Urethral Specimens for Chlamydial Infections PCR-polymerase chain reaction. TMA-transcription mediated amplification. SDA-strand displacement amplification. Cook RL, et al. Annals Int Med. 2005; 142: 914

39. Performance of an Amplification Test for the Detection of N. gonorrhoeae in Endocervical, Male Urethra and Urine Specimens *True positive defined as positive endocervical or urethral culture or LCR and alternative gene target positive by LCR Koumans EH, et al. Clin Infect Dis 1998;27:1171.

40. Potential Urine Based Screening Opportunities • Juvenile detention/jails • Emergency departments • High schools • General medicine clinics, especially teen clinics • Drug rehabilitation programs • Homeless clinics • Military induction centers

43. 1 2 3 Carefully put the swab about two inches inside the opening of your vagina and gently turn the swab for 20 seconds. Make sure the swab touches the walls of your vagina Take the swab out of the sealed package Remove the swab and hold it in the middle of the plastic handle 5 4 6 Carefully break the plastic handle against the side of the tube Remove the cap from the tube and put the swab into the tube so that you can see the tip is below the label Tightly screw the cap onto the tube. Follow the packaging instructions and mail the specimen Vaginal Swab Study Specimen Collection Illustration

44. Sensitivity of Different Specimens by Three Different Assays FCU – first catch urine, Cx – endocervix, S-vag – self-collected vaginal swab, C-vag - Clinician collected vaginal swab. Schachter J, et al. STD 32;2005:725

45. Testing Preference Survey Among 1090 Women Following a Pelvic Examination and a SOVs Protocol • 90% found self collection of vaginal swabs very easy and another 7% found it somewhat easy. • 76% preferred a SOVs over a pelvic examination. 60% preferred it over a urine specimen. • 94% said they would be tested for STIs more often is SOVs were available. Chernesky MA, et al. Sex Transm Dis 32; 2005:729

46. Penicillin and Tetracycline Resistance Among U.S. Gonococcal Isolates- 2000

47. 2006 CDC STD Treatment Guidelines Uncomplicated Gonococcal Infections • Recommended Regimens Cefixime 400 mg orally in a single dose or Ceftriaxone 125 mg IM in a single dose or Ciprofloxacin 500 mg orally in a single dose or Levofloxacin 250 mg orally in a single dose PLUS (If chlamydia not ruled out) Doxycycline 100 mg orally 2 times a day for 7 days or Azithromycin 1 gm orally

48. Percent of U.S. N. gonorrhoeae Isolates With Decreased Susceptibility or Resistance to Ciprofloxacin, 1990–2000

49. 2006 CDC STD Treatment Guidelines Uncomplicated Gonococcal Infections • Alternative Regimens Spectinomycin 2 g IM single dose Other single dose IM Cephalosporins (cefotaxime 500 mg, cefoxitin 2 g + probenecid, etc.) Other single dose Quinolones (enoxacin 400 mg, lomefloxacin 400 mg, norfloxacin 800 mg)

50. 2006 CDC STD Treatment Guidelines Chlamydial Infections • Recommended Regimens Azithromycin 1 gram, orally, single dose Doxycycline 100 mg orally 2 times a day for 7 days