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BRUCELLOSIS by

BRUCELLOSIS by. HASUTJI ENDAH NARUMI. DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGY FACULTY OF VETERINARY MEDICINE AIRLANGGA UNIVERSITY. PRIMER attack to cow, sheep and pig. SECUNDAIR attack to other animals and human being. COW Bang’s disease.

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BRUCELLOSIS by

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  1. BRUCELLOSIS by HASUTJI ENDAH NARUMI DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGY FACULTY OF VETERINARY MEDICINE AIRLANGGA UNIVERSITY

  2. PRIMER attack to cow, sheep and pig SECUNDAIR attack to other animals and human being COW Bang’s disease HUMAN undulans fever (malta fever) Brucellosis = Abortus Infections Disease

  3. ECONOMIC COST • Abortus • Give birth to meal • Reproduction disturbance infertility • Milk product x • Low mortality • Treatment value • Zoonosis (important)

  4. ETIOLOGY • - Stick - Gram (-) - Non motile - Non spora • Intra seluler • Three species which attack to livestock - B. militensis - B. abortus - B. suis

  5. Cow and dog B. abortus infections abortus • B. abortus attack horses, pigs • B. suis pigs, dogs • B. melitensis sheeps EPIZOOTIOLOGY Disseminating cow milk in Java Susceptible animal Rescue institute of animal disease Bekasi, Bogor, Kediri & Jakarta Health office human which work at Denpasar

  6. PATHOGENESIS The transmission of disease Through • Gastrointestinal tract • Genitalia tract • Skin, mucosa

  7. Infectious abortus temporary / permanent of 5 - 8 months • Occur revers to normal • milk product • Health produce infectious vaginal liquor • Mindness of foetal liquor • Pituitary gland ≠ symptom, other side milk contaminated againts bacteri nipple zoonosis • Epidydimitis (Male Epidydimitis) & orchitis SYMPTOM OF THE DISEASE Clinical symptom

  8. 2. POST MORTEM • Thickening of placenta • Spreads over the chorions layers • Mindness of foetal of liquor, brown - yellow, mixed with pus

  9. Male : Testis Inppurate Necrose Removal and Materials Delivery • Put into ice (suggestion) • Delivery to the lab • Put on preserved solution • Materials : • Milk : all nipple / not all nipples contaminated with bacteria • Vol 20 ml < washing with alc sol 70% • 1st, 2nd out pouring should be throw among • In the cold condition

  10. Vagina : removal on 6 weeks post partum / abortus Using wire, with cotton point (absorb a thread) put the cotton in to the glass jar Blood cultivate (from V. Jugularis) - This area should be wash and rubbed this area using cotton talk 70%. - Put 10 ml with spuit + heparin (anti coagulant) Blood serologic test

  11. Placenta : infections cotyledone smears slide fictations using methanol sol Fetus abortus : - Disposal of gatric - Pulmo - Lien - Fetal disposal - Take with sterile pasteur pipet Carcass : - The predict of bact is different among the species animals - Cow lgl supramamaria, lien, nipple, uterus

  12. DIAGNOSIS - Material samples - Base on : • Clinical symptom • PA • Laboratory : - Isolated and bacteria identification - Serologic test, blood serum thrid of mucosa vagina / milk, sperm

  13. DIFFRENTIAL DIAGNOSA • Abortus : infection camphylobacter foetus and trichomonas foctos • C. Foetus abortus usually happen every time • T. Foetus abortus happen earhide • Brucella abortus happen after 6 months

  14. PREVENTIVE OF THE DISEASE Route of administration : • to classified among • report the sample • to give the certificate for free Brucellosis • to give a tag for each sample post vacc & reactor

  15. Sanitation : • To burn the infectious material • To avoid a matting which suffer from a disease • Avoid for young animals from mother which suffer from a disease to give milk • Crages should be disinfected to avoid suffer from a disease • To refreshment a animals should be free from brucellosis • To monitor the mobilitation or animals

  16. ERADICATION • Standardization Dx / for Brucell (methods and reagent) • To point out infect and healthy area from Brucellosis • To point out infections cattles and healthy area from brucella • To point a policy for use a vaccination • To give a certificate of health for a healthy care

  17. Leptospirosis = Weil’s disease • Attacks animals and human • Cause of spirochaeta bact, genus of leptospira • The change subclinic of infectious were : abortion, hepatitis and nephritis death

  18. Resistant inside the water (1 month) • Inside the sea water, urine + rapidly ETHIOLOGY F o r m : • Long filament • Gram (-) • Aquatic saprofite apparently

  19. Isolation from human, dog, pig and micer / rat • Serolog reactor + cow, buffalo and pig • Pig at RPH Bogor, which contain bacteria inside the ren • At middle & East Java economic cost at the cattle of pig and cow EPIZOOTIOLOGI Incident in Indonesia

  20. Incident in Philippine

  21. The Familiar Of Several Leptospira • Leptospira canicola dog • Leptospira harjo cow • Leptospira ictero haemorrhagie mice / rat • Human infection through water, soil, flora which contaminated of animal urine • Incubation periods 4 - 19 days

  22. PATOGENESIS The Route of Disease Mechanisms • Bact body through mucosal layer multiple especially in : liver, ren, mammary gland, brain layer • Leptospiremia 1st week post infect • Bact produce toxin and hemolysin blood capilary disturbance tissue disturbances • Bact grow at tubulus renalis release via urine • Bact still alive in the urine several hour at 150C (several days at 40C) • Lethal because of septichemia, anemia, liver disorder and uremia • State of infect depend on : age, species, bact reservoir cause of infect.

  23. Human Infected From Contaiminated Animals (Rats) Bact inside the ren come out from urine • Cow (acute, subacute and chronic) • Acute : » fever, anemia, T after 2 - 4 days • » Milk prod become normal after 2 weeks • » Milk produce mixed with pus • Pig : » abortus • » retentio secundinarum • » + neonatal • » bact vehicle for cow & human CLINICAL SYMPTOM

  24. POSTMORTEM DISORDER • Cow : liver abses, ren colored black dark • Calf : icterus, liver absces, dark ren, petechial haemorrhagie, VU contain clean red urine • Chronic :absces ren, if slices contained of liquid

  25. DIAGNOSIS • Bacterial Isolation • Inject animal • Live animal under fever condition : - Urin without preserve - Aseptic blood + citrate - Aseptic milk and not preservative • From tetal animal - Urine, liver slices, ren, lien and blood cor • From healthy animals - Urine and serum Material of Sample

  26. Babesiosis with blood strike not effective againt of leptospirosis • Because the symtomp is abortus until confused with the cause of the other abortus • Clinical symptom, Hb uria, cause of clostridum, haemolyticum DIFFERENSIAL DIAGNOSIS

  27. PREVENTION • Death of animal - which bring the leptospira especially rodentia mild • R/ Antibiotics (Penicilline) L. canicola - Streptomycine, Aureomycine

  28. THANK YOU

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