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Microbial Source Tracking Techniques: Lake Michigan Beaches Case Studies. Erika Jensen, M.S. Great Lakes WATER Institute April 14, 2005. Potential Sources of E. coli. CSOs, SSOs, and septic systems Urban and agricultural runoff Waterfowl, domestic pets, and wildlife
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Microbial Source Tracking Techniques: Lake Michigan Beaches Case Studies Erika Jensen, M.S. Great Lakes WATER Institute April 14, 2005
Potential Sources of E. coli • CSOs, SSOs, and septic systems • Urban and agricultural runoff • Waterfowl, domestic pets, and wildlife • Sand, algae, and interstitial waters 250,000-500,000 CFU/100 ml Beach closure 235 CFU/100ml 240,000 CFU/100 ml 10,000-100,000 CFU/100 ml 368,000,000 CFU/g feces
GLWI Research Areas Fate and transport of bacteria Milwaukee Harbor, stormwater, Lake Michigan Sources of pollution Milwaukee Harbor, stormwater, Lake Michigan Door, Manitowoc, Milwaukee, & Racine County Beaches Pathogen occurrence Bradford Beach, Racine, Milwaukee Harbor Evaluation of BMPs South Shore beach, green roofs and rain gardens
Approach to Microbial Source Tracking • Gather the experts and share knowledge • In depth spatial surveys • 3. Targeted sampling to observe dynamics; modeling • 4. Apply source tracking approaches: • Human vs. non-human? • Are specific groups of animals contributing? • Does sand or Cladophora act as a reservoir? • 5. Manage and evaluate the problem
Milwaukee River Basin • 900 sq mi watershed • 410 miles of streams • 1.3 million people • Urban, industrial, • agricultural land uses
Bacterial Surveys Spatial & Temporal South Shore Beach Study: 2001 to present Menomonee River Study: 2002 Fate and Transport Study: 2002 to present Manitowoc & Door County Beach Surveys: 2002 - 2003 Bradford Beach Study: 2004 to present
Spatial surveys 2001-2004 Rain, N wind No rain – SE Wind CSO - 36 hrs, SE wind CSO - 45 hrs, SE wind 2001
Dry weather (baseflow) Wet weather (stormflow) rivers rivers
Menomonee River Study 2002 • Determine river E. coli levels during baseflow and stormflow • Determine E. coli levels of inline stormwater entering river • Characterize “genetic profile” of E. coli in stormwater
Menomonee River Survey 1 2 3 4 5 6 7 8 9 10 Suburban, suburban industrial Natural bed Urban, industrial Natural bed Urban, Rehabilitated bed Urban, Concrete bed
Fate & Transport CSO Event May, 14 2004
Hydrodynamic Model • Hydrodynamics • - Transport • - Mixing • - Dilution • Bacterial counts
Bacterial Die off in Lake Michigan • Die-off is a 2nd order function • Die off in Lake Michigan is 90% in 6-8 hours
E. coli Survival & Persistence in the Environment Pre-rain 6-14-04 09:44 Post-rain 6-14-04 10:20
E. Coli in Sand Range 8-39,000 E. coli/100 g
1 2 3 Clonal Populations Suggest Growth E. Coli from Beach Sand
Bacteroides spp. • Case Studies • Stormwater • Beach water • Agricultural Runoff • Combined Sewer Overflows
Summary • Spatial surveys provide the most useful data Identify hot spots or areas of concern • Implement targeted sampling surveys to observe site specific dynamics • Apply source tracking approaches: Human vs. non-human? Are specific hosts contributing? Are there environmental reservoirs? • Manage and evaluate the problem
Great Lakes WATER Institute P.I., Dr. Sandra McLellan Researchers Annette Daniels Alissa Salmore Caitlin Scopel Michelle Luebke Pat Bower Ola Olapade Graduate Students Marcia Silva Sachie Owaga Heidi Pirkov Liang Peng Sukpreet Kaur Students Magnolia Tulod Josh Harris Elissa Lewis Emerson Lee Jennifer Lee Andrew Holland Becky Kirby Hilary Street Ben Weston Morgan Depas Meredith Van Dyke Funding kindly provided by Milwaukee Metropolitan Sewage District Wisconsin DNR National Institute of Health NOAA Sea Grant SC Johnson Wisconsin Coastal Management Program