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Hemocompatibility of Plasma Treated Si Incorporated Diamond-like Carbon Films

Hemocompatibility of Plasma Treated Si Incorporated Diamond-like Carbon Films. R. K. Roy, M.-W. Moon, K.-R. Lee Future Convergence Research Laboratories, KIST, Seoul, Korea D.K. Han Biomaterials Research Center, KIST, Seoul, Korea J.-H. Shin

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Hemocompatibility of Plasma Treated Si Incorporated Diamond-like Carbon Films

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  1. Hemocompatibility of Plasma Treated Si Incorporated Diamond-like Carbon Films • R. K. Roy, M.-W. Moon, K.-R. Lee • Future Convergence Research Laboratories, KIST, Seoul, Korea • D.K. Han • Biomaterials Research Center, KIST, Seoul, Korea • J.-H. Shin • Department of Radiology, Asan Medical Center, Universtiy of Ulsan, Korea • KamijoUniv. Tokyo Hospital, Tokyo, Japan • T. Hasebe • Tachikawa Hospital, Keio University, Tokyo, Japan ICMCTF 2008, San Diego, USA

  2. Bioimplant Materials • Requirements for Bioimplants • Should not cause infections • Prevent uncontrolled cell growth • Maintain their integrity inside the body • Interact in a controllable way with the biological environment • Avoid formation of debris Surface Properties

  3. Diamond-like Carbon : as a Strong Candidate Coating DLC for biomaterials • Biological Compatibility • Nontoxic, Noncarcinogenic, Noninflammatory • Chemical Compatibility • Corrosion Resistance • Mechanical Compatibility • Surface Hardness, Wear Resistance

  4. Hemocompatible and Hermetic Coating Vascular Stents • Suppress the formation of blood clots • Prevent the release of metal ions Clotted Artery

  5. CarbofilmTM by Sorin Biomedica, Inc. DLC Coated Blood Contacting Implants

  6. Systematic study on the effect of surface properties on the hemocompatibility. The present work • Plasma treatment of Si-DLC coating • Characterization of the surface • Wetting behavior • Surface chemical bonds • Hemocompatibility tests • Protein adsorption (Albumin/Fibrinogen ratio) • Activated Partial Thromboplastin time • Platelet adhesion and activation

  7. Si-DLC Film Potentiodynamic Polarization in Saline Solution Thin Solid Films, 475, 291-397 (2005). J. Biomed. Mater. Res. A in press (2007).

  8. Film Preparation • Film Deposition • C6H6 + SiH4 • Pressure : 1.33 Pa • Bias voltage : -400V • Film thickness : ~500nm • Si Concentration in the film : 2 at.% • Surface Treatment • O2, N2, H2, CF4 • Pressure : 1.33 Pa • Bias voltage : -400V • 10min Schematics of RF PACVD system.

  9. Surface modification of Si-DLC

  10. q Energetics of Surface

  11. Surface Energy

  12. Interfacial Tension with Human Blood

  13. XPS Anaysis

  14. XPS Analysis

  15. XPS Anaysis Si-DLC (O2) Si-DLC Si-DLC (CF4)

  16. XPS Anaysis Si-DLC Si-DLC (N2)

  17. Plasma Protein Adsorption • Better hemocompatibility can be expected on the surface with higher ratio of albumin/fibrinogen adsorption. • ELISA analysis after treating the samples with albumin (3mg/ml) and fibrinogen (0.2mg/ml) solution.

  18. aPTT Measurement • Activated partial thromboplastin time (aPTT) determines the ability of blood to coagulate through the intrinsic coagulation mechanism. The longer aPTT time is obtained on better hemocompatible surface. • Soaking for 60min in platelet poor plasma (PPP: 7x103/ml) using human whole blood from healthy volunteer.

  19. Platelet Adhesion Measurement • Soaked for 60 min in PRP (1.5x105/ml) from human whole blood from healthy volunteer. • Adherent platelet are fixed and dehydrated for observation under OM and SEM.

  20. Platelet Activation Goodman and Allen et al. Lose discoid shape Develope thin pseudopodia Become large, spiny sphere covered by pseudopodia On a-C:H surface Fully spread

  21. Platelets on Si-DLC

  22. Platelets on Si-DLC (N2)

  23. Platelet on Si-DLC (O2)

  24. Nitrogen or Oxygen Plasma Treatment

  25. XPS Analysis

  26. Which surface bond is significant?

  27. Conclusions • Hemocompatibility of Si-DLC film was improved by the surface treatment using nitrogen and oxygen plasma. • Large surface energy (large polar component) • Low interfacial energy with blood • Both C-O and Si-O bonds on the plasma treated Si-DLC surface play a significant role in improving the hemocomptatibility. R. K. Roy et al,Diam. Rel. Mater (2007). Submitted to Acta Biomater. (2008).

  28. Acknowledgement Financial Support from 'Center for Nanostructured Materials Technology' under '21st Century Frontier R&D Programs' of the Ministry of Science and Technology of Korea (code #: 06K1501-01610), and Taewoong Medical Co. Ltd.

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