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Fig.3. A. 4 h-treatment. 10 min-treatment. 40. Non-permeabilized Jurkat T cells. + rIgG 1 13B8.2. 11%. + rIgG 1 13B8.2. Events. 10 2. 10 2. 10 1. 10 0. 10 1. 10 0. 21%. 0. Permeabilized Jurkat T cells. 40. + rIgG 1 13B8.2. + rIgG 1 13B8.2. 50%. Events. 73%. 10 2.
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Fig.3 A 4h-treatment 10 min-treatment 40 Non-permeabilized Jurkat T cells + rIgG1 13B8.2 11% + rIgG1 13B8.2 Events 102 102 101 100 101 100 21% 0 Permeabilized Jurkat T cells 40 + rIgG1 13B8.2 + rIgG1 13B8.2 50% Events 73% 102 101 100 0 102 101 100 Anti-ceramide antibody B 50 40 30 Ceramide-positive Jurkat T cells (%) 20 10 0 Inhibitor - - FB1 FB1 Imi Imi Myr Myr rIgG1 13B8.2 - + - + - + - +