1 / 8

Photosynthesis

Photosynthesis. Photosynthesis. light reactions (thylakoid) 6CO 2 + 12 H 2 O gives C 6 H 12 O 6 + 6O 2 + 6H 2 O Calvin cycle or dark reactions (stroma)

tegan
Download Presentation

Photosynthesis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Photosynthesis

  2. Photosynthesis • light reactions (thylakoid) • 6CO2 + 12 H2O gives C6H12O6 + 6O2 + 6H2O • Calvin cycle or dark reactions (stroma) • 􀁡photoenergy is required (endergonic

  3. Using the Spectroscope • Using the Spectroscope • 􀁡view white light w/ spectroscope,record colors seen • 􀁡view red, blue, and green filters w/ • Spectroscope,record colors seen • 􀁡view spinach extract w/ spectroscope,record colors seen

  4. Using the Spectrophotometer • tube 1 spinach extract • tube 2 (blank) solvent • tube 3 carrot extract • 􀁡 record %T from 400-700 nm in 25nm • increments, graph • 􀁡 100% T must be reset whenever λ is • changed % %

  5. Chromatography • solvent line • carotenes = orange • carotenoids • xanthophylls = yellow • chlorophyll a = blue-green chlorophylls • chlorophyll b = yellow-green • baseline

  6. Chromatography, Rf values • Chromatography, Rf values • 􀁡Rf = pigment distance/solvent distance • 􀁡affinity for the solvent = high Rf value • 􀁡affinity for the paper = low Rf value • 􀁡Staple finished chromatograms to report

  7. Starch localization in leaves • Starch localization in leaves • 􀁡Boiling the leaf in alcohol will remove the • pigments, it will turn white • 􀁡flood leaf w/ iodine • iodine + starch = blue-black • Draw leaf in color before boiling and after staining.

  8. Chloroplast Model • Label chloroplast in your lab manual • 􀁡outer membrane • 􀁡inner membrane • 􀁡intermembrane space • 􀁡stroma (Calvin cycle or dark reactions) • 􀁡thylakoid (light reactions), granum, grana

More Related