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Status Report

Status Report . By: SJ DeGroote and Sarah Pellerino. Background Info. A phage is a virus that infects and replicates inside bacteria. They cannot live on their own and they depend on a host cell to replicate and spread.

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Status Report

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  1. Status Report By: SJ DeGroote and Sarah Pellerino

  2. Background Info • A phage is a virus that infects and replicates inside bacteria. • They cannot live on their own and they depend on a host cell to replicate and spread. • For a long time phages and other viruses weren’t studied and were basically completely ignored, but it’s starting to come to attention that viruses are actually an important part of life and need to be studied and researched.

  3. DeGroovy Jr. • From a straw bale garden with plants growing in them in River Falls township, Wisc. on 9/19/13 at 7:15am. Air temp. was 62 degrees F. Cloudy and humid. • Various sizes ranging from 1mm to 3mm • Clear, tiny halo • HTL titer: • SJ: 3.0 x 10^8 pfu/ml • Sarah: 1.2 x 10^5 pfu/ml Figure 1 (above) Figure 2 (right)

  4. SJ DNA Isolation HindIII HaeIII EcoRI ClaI BamHI Undig. dna Kb • Concentration: 0.0775μg/μl • Yield: 7.75 μg • A260: 0.155 • A280: 0.089 • Ratio: 1.742 Figure 3 First DNA gel results

  5. Sarah DNA Isolation HindIII HaeIII EcoRI ClaI BamHI Undig.Dna Kb • Concentration: 0.0985 μg/μl • Yield: 9.85 μg • A260: 0.197 • A280: 0.112 • Ratio: 1.759 Figure 4: First DNA gel results

  6. SJ’s are the top four wells, Sarah’s are the bottom four wells. Pst 1 Sal 1 Eco Rv Nco 1 Kb Pst 1 Sal 1 Eco Rv Nco 1 Figure 5 second DNA gel results

  7. Electron Microscope Sarah Avg. head size: 40nm Avg. tail size: 90nm SJ Avg. head size: 41.6nm Avg. tail size: 93.75nm Figure 6 SJ’s electron microscopy Figure 7 Sarah’s electron microscopy

  8. Similarities PstI Sal I Eco Rv Nco I Kb Nco I Eco Rv Sal I Pst I • Jessica Dahlke and Emma Sweet’s second DNA gel run seem to be identical to our second gel. Although, the average head and tail sizes from the electron microscopy differ, so we’re not certain if they are the same or not. Figure 8 Jessica and Emma’s 2nd DNA gel

  9. References • Kirby, Breeann, and Jeremy J. Barr. “Going Viral.” The Scientist 1 Sept. 2013:1-6. The Scientist. Web. 2 Dec. 2013.

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