28S rRNA

1. SL-5’UTR control – 30’ SL-5’UTR control – 0’ SL-AUG control – 30’ SL-AUG control – 0’ 5’UTR control – 30’ 5’UTR control – 0’ AUG control – 30’ AUG control – 0’ 28S rRNA 16S rRNA S1: Homogeneity and stability of the mRNA translated: A - Homogeneity and stability of the mRNA constructs in the RRL. 0.05 µM of 32P labeled 5’UTR Control, SL-5’UTR Control, AUGControl, SL-AUG control were incubated for 0 or 30 minutes in 15 µL RRL under translational conditions. The reaction was stopped on ice in the presence of 1% SDS, phenol extracted, ethanol precipitated, and the RNAs analysed on a 1% denaturing agarose gel. The 18S and 28S rRNA were visualized by SyBr staining before autoradiography and are indicated on the left hand side of the figure.

2. SL-HIV-2-5’UTR –30’ SL-HIV-2-AUG1 – 30’ SL-HIV-2-5’UTR – 0’ SL-HIV-2-AUG1 – 0’ HIV-2-5’UTR – 30’ HIV-2-5’UTR – 0’ HIV-2-AUG1 –30’ HIV-2-AUG1 – 0’ 28S rRNA 16S rRNA B - Homogeneity and stability of the mRNA constructs in the RRL. 0.05 µM of 32P labeled HIV-2-5’UTR, SL-HIV-2-5’UTR, HIV-2-AUG1, SL-HIV-2-AUG1 were incubated for 0 or 30 minutes in 15 µL RRL under translational conditions. The reaction was stopped on ice in the presence of 1% SDS, phenol extracted, ethanol precipitated, and the RNAs analysed on a 1% denaturing agarose gel. The 18S and 28S rRNA were visualized by SyBr staining before autoradiography and are indicated on the left hand side of the figure.