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Label-Free Bioanalytical Detection Using Membrane-Coated Silica Nanoparticles

Label-Free Bioanalytical Detection Using Membrane-Coated Silica Nanoparticles Michael M. Baksh, Esther M. Winter, Nathan G. Clack and Jay T. Groves: University of California, Berkeley and Lawrence Berkeley National Lab Stanford MRSEC, DMR-0213618. 15µm.

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Label-Free Bioanalytical Detection Using Membrane-Coated Silica Nanoparticles

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  1. Label-Free Bioanalytical Detection Using Membrane-Coated Silica Nanoparticles Michael M. Baksh, Esther M. Winter, Nathan G. Clack and Jay T. Groves: University of California, Berkeley and Lawrence Berkeley National Lab Stanford MRSEC, DMR-0213618 15µm • Membrane-coated silica particles exhibit colloidal phase transitions that are governed by membrane surface interactions.  • Collective phase behavior of the beads serves as a cooperative amplifier; a readily detectable response from small numbers of microscopic binding events between ligands and membrane-bound protein(s) of interest alters the structure of the colloidal dispersion in measurable ways. • Further statistical analysis of bead pair distribution functions enables quantitative determination of binding affinities. • This technology has already been patented, and is currently being licensed by NuvoMetrix Inc. for commercialization. • Beads spontaneously form two-dimensional structures. • Silica microbeads are coated with synthetic or natural membranes containing ligand of interest. • Beads are allowed to settle to the bottom of a well. • Radial distribution function (to measure changes in colloidal structure) varies continuously with the concentration of ligand bound to the bead surface. Baksh, Jaros, & Groves, Nature 427:p.139-141 (2004)Winter & Groves, Anal, Chem 78:p. 174-180 (2006)

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