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Laboratory Filtration

Laboratory Filtration. Cassidy Markee Director of Laboratory Division. Spectrum’s Core Technology: Membrane Separation. Spectrum offers products for two kinds of Membrane Separation (membrane, systems, components and accessories):

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Laboratory Filtration

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  1. Laboratory Filtration Cassidy Markee Director of Laboratory Division

  2. Spectrum’s Core Technology: Membrane Separation • Spectrum offers products for two kinds of Membrane Separation (membrane, systems, components and accessories): • Membrane Dialysis – passive diffusion through a semi-permeable membrane – 100 to 1,000,000 Daltons • Membrane Filtration – applied pressure across a selective membrane. 3 types based on size range: • Macrofiltration – 5 μm and up • (Woven Mesh Screens) • Microfiltration – 0.05 – 5 μm • (Direct Flow and Tangential Flow) • Ultrafiltration (Molecular filtration) – 5 kD to 0.05 μm • (Tangential Flow only)

  3. Membrane Microfiltration in the Lab Two membrane configurations for Direct Flow Filtration (DFF): • Flat sheet DFF Devices - disadvantages • Less surface area - lower flux rate • Quicker to plug or foul • Lower particle load • Loped Hollow Fiber DFF Devices - advantages • Higher surface area - higher flux rate • Longer filter life – less fouling • High particle load

  4. Mode of Action: Flatsheet DFF

  5. Mode of Action: Hollow Fiber DFF

  6. Spectrum’s Hollow Fiber DFF Devices Features & Benefits: • Sterilizing grade 0.2 um Mixed Cellulose Ester (ME) - DynaFibre® • Looped hollow fiber membrane provides large surface area • Higher flux rates and increase filtration volume • Filtration from outside fiber into lumen reduces fouling and increases particle load • Compact and narrow filter housing • PP air vent fiber loops prevents “air lock”

  7. Hollow Fiber Microfiltration: 4 Types of Filters MediaKap® & MediaKap® Plus Cold sterilization of culture medium CultureGard® Sterile barrier for feeding/perfusion of bioreactors DynaGard® Syringe tip filtration MiniKap® Point-of-use water filtration

  8. DynaGard® Syringe Tip Filters • Aqueous solution filters (blue) • 0.2 μm Cellulose Ester Membrane • 3 sizes for 1-20 ml • Organic solution filters (white) • 0.2 & 0.45 μm Polypropylene Membrane • 2 sizes for 1-20 ml • Low fouling • Irradiated or non-irradiated • Narrow housing design • Luer-lok™ connections

  9. MediaKap® & MediaKap® Plus HF Filters • MediaKap for serum-free media • MediaKap Plus for serum-enriched media • Skinned DynaFibre for higher viscosity • 2x filtration capacity for serum-free media • 0.2 um sterilizing grade cellulose ester membrane • 5 sizes – 2, 5, 10, 25 & 50 liters • 0.2L – 100L (400ml/min – 2L/min) • Low extractable • Optional filling bell– protects sterility • PP vent fiber prevents air lock

  10. CultureGard® HF Perfusion Filters • Sterile protection during culture perfusion, feeding or sampling • Large surface area • Minimal hold-up volume • PP vent fiber prevents air lock • Process highly viscous solutions • Use two in series to increase duration of feeding

  11. MiniKap® Water and Gas Filters • Point-of-Use aqueous final filter • Removes particles in air/gas lines as low as 0.01 μm • Validated for use in clean rooms • Industries served: • Electronics/Semiconductors • Medical Device Manufacturing • Biotech/BioPharm • Petrochemical

  12. Thank You谢谢

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