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Chemiluminescent ELISA Kits What is CDSimple™ Chemiluminescent ELISA? CDSimple™ Chemiluminescent ELISA kits use a chemiluminescent substrate for detecting key biomarkers in life science research and requires a luminometer for output reading. These kits save time and money with highly sensitive and accurate broad range assays in comparison immunoassays. to standard colorimetric How does CDSimple™ Chemiluminescent ELISA work? The assay employs enzymatic chemiluminescence sandwich immunoassay technique. The immobilization takes place during the assay at the surface of a magnetic beads or plates through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal antibody. Upon mixing monoclonal biotinylated antibody, the enzyme labelled antibody and a serum containing the native antigen, a reaction results between the native antigen and the antibodies, without competition or steric hindrance, to form a soluble sandwich complex. Simultaneously, the complex is deposited to the streptavidin plates or magnetic beads through the high affinity reaction of streptavidin and biotinylated antibody. http://www.creative-diagnostics.com/cdsimple-chemiluminescent-elisa-kits.ht m