ABSTRACT

1. (b) (a) (c) (d) MODERN THERAPIES BASED ON NANOBIOTECHNOLOGY IN PSORIAZIS Adriana Vulcu1 , Liliana Olenic1, Simina Dreve1, Luminita David2, Bianca Moldovan2, Maria Perde-Schrepler3, Adriana Gabriela Filip4 , Maria Crisan4, Ioana Chiorean5 1National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania 2Faculty of Chemistry and Chemical Engineering, 11 Arany Janos Street RO 400028, Cluj-Napoca, Romania 3“Ion Chiricuţă” Oncology Institute, 34-36 Republicii Street Cluj-Napoca, Romania 4Faculty of General Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Strada Emil Isac 13, Cluj-Napoca, Romania 5Faculty of Mathematics and Informatics, , Babeş-Bolyai –University, Kogălniceanu Street, 1, Cluj-Napoca, Romania 400084 INCDTIM • ABSTRACT • Silver nanoparticles (AgNPs) have potential application as vehicle for some organic compounds transported through membranes; • Anthocyanins are polyphenolic compounds found in flowers, fruits and vegetables and are widely studied for their antioxidative,and antiinflammatory effects • The aim of this study is to investigate the biological activity of noble metal nanoparticles functionalized with anthocyanins extracted from Adoxaceae family (Viburnum opulusL – E1 and Sambucusnigra L. – E2) • Due to the fact that the psoriasis, chronic inflammatory disease that affects mainly the skin, is a continuous challenge for researchers, effectiveness of topical therapies and nanotechnology based anthocyanins in psoriasis therapy was taken into account in a clinical study. EXPERIMENTAL Nanomaterials preparation and characterization Figure 1. (a) Viburnum opulus L., berries; (b) Sambucus Nigra L., berries;(c) And (d) TEM images of AgNPs with E1 espectively E2 Figure 2. UV-Vis spectra of (a) AgNPs-E1; (b) AgNPs-E2 RESULTS AND DISCUSSIONS 2. In vivo toxicity studies 3. Evaluation of new prepared nanomaterials on skin diseases 1. In vitro toxicity studies Figure 5. Seric malondialdehyde level at lots with induced inflammation and treated with NP functionalized with anthocyanins Figure 8. Psoriasis plaque (a) before treatment; (b) after treatment (c) sonogram skin with Dermascan Ultrasound 20MHz Figure 3. The effect of AgNPs-E1 (a,b) and AgNPs-E2 (c,d) on the viability of HaCaT cell lines (Keratinocytes cells) and A431 cells (epidermoid carcinoma cells) Figure 6. Glutathion level at lots with experimental induced inflammation and treated with NP functionalized with anthocyanins Figure 9. Values of dermis thickness before therapy Figure 7. Foot edema at 2h after injection with carageenan * p <0.05 between the group treated with diclofenac and treated with anthocyanin; ** p <0.01 between the group treated with diclofenac and treated with AuNPs functionalized with anthocyanins and between groups treated with Ag/AuNP functionalized with anthocyanins Figure 10. Values of dermis thickness after therapy Figure 4. Comparative effect of AgNPs-E1 and AgNPs-E2 on cellular viability. AgNPs-E2 show higher toxicity in both cell lines • CONCLUSION • Silver nanoparticles functionalized with anthocyanins extracted from Viburnum opulusL and Sambucusnigra L. (AgNPs-E1 and AgNPs-E2) were prepared and characterized; • The viability of cells decreased dose-dependly, and was significant in both cell lines (HaCaT and A431) at the dose 47.5 μg/ml for AgNPs-E1 and at 2.37 μg/ml for HaCaT, while for A431 was 95 μg/ml for AgNPs-E2; • Oral administration of AgNPs functionalized with anthocyanins has antiinflammatory effect similar with diclofenac administration; • Anthocyanins influence the structure of psoriasis plaque due to the anti-inflammatory effect, possibly augmented by nanotechnologies. The therapy is promising for future investigations to prove the anti-psoriatic activity. Acknowledgement This work was elaborated in the frame project no 147/2012, through the program “Partenerships in priority areas-PN II”, developed with the support of ANCS, CNDI-UEFISCDI