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Johnny Yin 18 April, 2014

Expert of yeast origin nutrients for “Microorganisms”. Johnny Yin 18 April, 2014. Contents. 1. 1. Introduction about Angel yeast Co.,Ltd. 2. Angel yeast extracts – Good nutrient-sources for microoganisms. 3. Application of Angel yeast extract.

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Johnny Yin 18 April, 2014

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  1. Expertof yeast origin nutrients for “Microorganisms” Johnny Yin 18 April, 2014

  2. Contents 1 1 Introduction about Angel yeast Co.,Ltd. 2 Angel yeast extracts – Good nutrient-sources for microoganisms 3 Application of Angel yeast extract

  3. 1. Introduction of Angel Yeast Co.,Ltd. 1.1 About us > 1.2 Global net work > 1.3 Our history > 1.4 Main business > 1.5 Angel’s value chain > 1.6 Employees > 1.7 Advantages >

  4. 1.1 About us • Angel yeast, founded in 1986, is a listed high-tech yeast company in China. • It is one of the largest global yeastand yeast extract manufacturers with 12 factories in China and Egypt up to now.

  5. 1.2 Global network 12 4 Factories Yeast extract plants 12 140 Technical centers Countries

  6. 1.3 History Project started Public company No.1 in Asia 1986 1989 2003 2008 Today 2000 First yeast extract facilities First facilities Global supplier

  7. The fastest growing company in global yeast industry CAGR 2011-2012 Angel yeast is estimated to grow at a CAGR of 20% 2011-2012, while the global yeast market’s CAGR is to be 8.6% (Foodnavigator.com, 2012-04-05).

  8. 1.4 Main business Main business • Bakery • Yeast extract-savory • Yeast extract-fermentation • Beverage & ethanol • Human health • Enzymes • Animal nutrition

  9. 1.5 Angel’s Value Chain Lab Yeast extract Inactive yeast Technical support Experiment Microorganism Yeast extract Industrial fermentation Package Trading Product Brewing Food Agriculture Environment Chemical Feed Energy Pharm Alcohol yeast Wine yeast Bakery Enzyme Seasoning Inactive yeast Yeast extract Yeast extract Bio-fertilizer Enzyme Enzyme Active yeast Yeast extract Inactive yeast Enzyme Yeast extract Yeast cell wall Feed yeast Yeast extract

  10. Our global partners

  11. 1.6 Employees> He Chongyue (Taiwan, China) Jan Hille ((Netherlands) Rosenberg (Netherlands) Tilak (U.S.) Yao Juan Li Xiao Kuang Jinbao Li Pei Li Zhijun 1331 employees, 70% of which are professional people Over 100 researchers work together with universities. 7 international experts employed as senior technical consultants.

  12. 1.7 Advantages 1.7.1 Strong production output > 1.7.2 Professional production process> 1.7.3 R&D strength > 1.7.4 Quality, safety and consistency > 1.7.5 Certification> 1.7.6 Environment protection > 1.7.7 Global professional service>

  13. 1.7.1 Strong production output > Total output of Yeast is 120.000 tons, Yeast Extract is 46.000 tons. Yichang Plant Yili Plant Chongzuo Plant BinzhouPlant

  14. 1.7.2 Professional production process

  15. 1.7.3 R&D strength > • 4 sub-headquarters with technical centers • National yeast technology research and promotion center • Post-doctoral scientific research workstation • R & D team led by PhDs and experts • National recognized laboratory CNAS • Basic research and Application research laboratory • Own the first-class test device and pilot plant.

  16. 1.7.4 Quality, safety and consistency > EU standard design production line Stable and consistent quality applies to all production plants Distributed control system Siemens PCS7 process control system Whole-course CIP cleaning SAP-ERP Management flat

  17. 1.7.5 Certifications > HALAL BRC SEDEX KOSHER(OU) CNAS ISO9001 ISO14001 ISO22000

  18. 1.7.6 Environment protection > First class environment protection facilities in each plant The high-concentration organic waste water was made into bio-organic fertilizer.

  19. 1.7.7 Global professional service> We do not only provide the best quality products, we also provide technical solutions for all our serving industries Set up global baking centers Over 200 application engineers

  20. 2. Angel Yeast Extracts – Good nutrient-sources for microoganisms 2.1 What’s yeast extract > 2.2 Why choose yeast extract for fermentation industry > 2.3 Range of Angel’s typical products >

  21. 2.1 What’s yeast extract? • Yeast extract is a kind of : • 100% animal free natural ingredient that is made from yeast. • High performance and stable sources of nutrients for microorganisms • Clean and safe raw materials for industrial fermentation and culture media

  22. Process of yeast extract production

  23. 2.1.1 Molecular weight distribution

  24. 2.1.2 Profile of Amino acids

  25. 2.1.3 Profile of free Amino acids and Total amino acids Free Amino acids / Total Amino acids ×100

  26. 2.1.4 Trace elements

  27. 2.1.5 Vitamins

  28. 2.1.6 Nucleic acid and Nucleotides

  29. 2.1.7 Functionality of yeast extract • Increase biomass • Improve the accumulation of metabolites

  30. 2.1.8 Stability of yeast extract Two key indicators: Total nitrogen and Amino nitrogen, For example: Stability of Yeast extract FM902

  31. 2.2 Why we choose yeast extract for fermentation industry? • Clean and Safe • - Clean and safe natural material for culture media and industrial fermentation • - Animal free, no risks of illness • - GMO free, Non allergen • - Kosher and Halal certified. • Stable quality • - Produced with strict quality control system. • - Large industry scale production • - No quality change in different seasons • High performance • - Advanced production technology • Beside yeast extract, Angel also produce yeast peptone and inactive yeast for fermentation industry

  32. 2.3 Range of Angel’s typical products

  33. FM888 Products FP101 Which are the most popular products in lab research?

  34. Angel Microfiltered yeast extract FM888 • Features and Advantages - High solubility - Easily assimilated by microorganisms and cells - Rich in peptides and growth factors - Low impurities • Application - Lab research Dehydrated culture media, Cell culture, Diagnostic media - High value-added industrial fermentation Insulins, Hormones, Hyaluronic acids

  35. Angel yeast peptone FP101 • Features and Advantages - Good replacement of Animal origin peptone - Could be combined with yeast extract - Rich in peptides • Application - Lab research Microorganisms culture, small scale fermentation - Industrial fermentation Probiotics, Enzymes, Hormones, Organic acids

  36. FM902 FM808 Products LD00 LM800 Which are the most popular products in industrial fermentation?

  37. Angel standard yeast extract FM808 • Features and Advantages - Good solubility - High nitrogen level - Rich in peptides - Light color • Application - Lab research Dehydrated culture media - Industrial fermentation Insulins, Hormones, Hyaluronic acids, Probiotics

  38. Angel standard yeast extract FM902 • Features and Advantages - Total soluble in water - High nitrogen level - Cost effective - High consistency • Application - Industrial fermentation For >60 types of fermentation-based products

  39. Angel standard yeast extract FM902

  40. Angel standard yeast extract FM902

  41. Angel standard yeast extract FM902

  42. Angel standard yeast extract FM902 Amino acid Enzyme Hormone Organic acid Probiotic Nucleotides / Nucleosides Poly saccharide Biological preservatives Antibiotic Industrial fermentation Mashroom Rhizobia Biological bait Effective Microorganisms Bio-fertilizer FM902 Agriculture Environment Waste water treatment Environmental microorganisms

  43. Angel standard yeast extract LM800 and LD00 • Features and Advantages - Paste and Liquid form of yeast extract FM902 - Rich in peptides - Cost effective - High consistency • Application - Industrial fermentation For >60 types of fermentation-based products

  44. How to judge the quality?

  45. 3. Application of Angel Yeast Extract 3.1 Summary of Angel yeast extract application > 3.2 Some experiments of Angel yeast extract >

  46. 3.1Summary of Angel yeast extract application • Biomass products industry - Starter cultures and probiotics for human food products - Cultivation of mashroom and macrofungi Recommendation: FM808, FM803, FM505, FP101 • Culture media industry and lab reagent - Bench scale research - Dehydrated culture media, Diagnostics, Cell cuture Recommendation: FM888, FM808 • Bioactive molecules and chemicals industry - Amino acids and peptides - Organic acids, Vitamins, Nucleotides, Enzymes, Alcohol - Vitamins,Antibiotics, Hormones, Biological preservatives - Other types of large scale fermentation Recommendation: FM808, FM902, LM800, LD00, YP101 • Agricultural and environmental industry - Sewage treatment, Bio-pesticide, Bio-plastic - Probiotics for agriculture and environment Recommendation: FM902, FM801, FM701, LM815, LP101

  47. 3.2 Some experiments of Angel yeast extract 3.2.1 Morphology of 4 types of typical microorganisms > 3.2.2 Some experiments of Angel yeast extract >

  48. 3.2.1 Morphology of 4 types of typical microorganisms after using yeast extract and other type of organic nitrogen Streptomyces lincolnensis Lactobacillus bulgaricus B A B A Bacillus subtilis Acetobacterium A B A B “A”—Another type of organic nitrogen “B”—Angel yeast extract

  49. 3.2.2 Angel standard yeast extract FM808 for Lactobacillus thermophilus growth Strain: Lactobasillus thermophilus Medium: Yeast Extract FM808 0.3%，Glucose 2%, etc. pH: 6.5 Angel yeast extract FM808 Control Result: Yeast extract FM808 could improve the growth of Lactobacillus thermophilus well Angel yeast extract FM808 Control

  50. 3.2.3 Angel standard yeast extract FM902 for the growth of E.coli and Absidia coerulea The growth curve of E.coli, comparison of FM902 and Brewer’s yeast extract The conversion rate of HC(from RSA), Absidia coerulea, yeast extract FM902

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