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Gram Negative Bacilli

Gram Negative Bacilli. Family: Enterobacteriaceae Genus : Proteus Species : Proteus Mirabilis, Proteus Vulgaris. Genus : Proteus. General Characteristics: G – ve , pleomorphic bacilli . Non – lactose fermenter Ferment glucose and produce acid + gas . Facultative anaerobes.

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Gram Negative Bacilli

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  1. Gram Negative Bacilli Family: Enterobacteriaceae Genus : Proteus Species : Proteus Mirabilis, Proteus Vulgaris

  2. Genus : Proteus General Characteristics: G – ve, pleomorphic bacilli. Non – lactose fermenter Ferment glucose and produce acid + gas. Facultative anaerobes. Highly motile. Grow as swarms (waves) on blood agar (swarming phenomenon). Fishy smell on culture. Urease+ve N. f .of human GIT .

  3. Genus : Proteus Diseases:- UTI, Stone formation (alkaline urine) form stone. Wound infection. Pneumonia. Sepsis.

  4. Genus : Proteus Swarming inhibition:- 1.Medium with surface active agent: e.g. Bile salt in MacConkey agar. 2. Increases agar concentration (5%). Advantage: regarded as a diagnostic feature for Proteus. Disadvantage: can causes misdiagnosis for other bacteria.

  5. Genus : Proteus LABORATORY DIAGNOSIS:- • Specimensaccording to disease e.g. in UTI urine. • Gram stain  Gram’s negative bacilli. • Culture: *MacConkey’s agar non lactose Pale colonies.

  6. Laboratory diagnosis *Blood agar  Swarming.

  7. Laboratory diagnosis *Glucose sugar fermentation acid &gas • *Urea agar (urease test) Pink (+); Yellow (-). • Motility test positive  inverted Christmas tree • API 20E (biochemical test)

  8. Genus Pseudomonas Genus PseudomonasSpecies: Pseudomonas aeruginosa.

  9. Genus Pseudomonas GENERAL CHARACTERISTICS G-ve bacilli, non – lactose fermenter. Ferment glucose and produce acid only. Strictly aerobic, oxidase positive Fruity (sweet) smell on culture. (green apple smell) Produce water - soluble pigments: Pyocyanine Blue – green. Pyorubrin Red. Pyoviridin Fluorescence. Pyomelanin Black. Actively motile ,N. f. of soil, water, plants, hospitalenvironment(sinks, showers, respiratory equipments).

  10. Genus Pseudomonas Pseudomonas Pyocyanine Pigment

  11. Genus Pseudomonas Pseudomonas Pyocyanine Pigment

  12. Genus Pseudomonas TRANSMISSIONS: Ingestion of water & food. Contaminated medical equipments. Contamination of wounds. Person to person.

  13. Genus Pseudomonas Diseases: Community acquired infections: Skin (folliculitis), eye infection after trauma, osteomyelitis after trauma, pneumonia. Nosocomial infections: Wound infection. UTI. Burn infection. Otitisexterna. Pneumonia. Sepsis. Highly resistant to antibiotics and disinfectant.

  14. Genus Pseudomonas pseudomonas infection

  15. Genus Pseudomonas LABORATORY DIAGNOSIS:- 1-Specimens(according to the type of infection) e.g. wound swab…etc. 2-Gram’s stain cylinder, Gram’s negative bacilli. 3-Culture: Aerobic, 37C° on: a.) MacConkey’s agar Non lactose fermentation Pale colonies.

  16. Laboratory diagnosis b.) Nutrient agar  Demonstrate pigment production.

  17. Laboratory diagnosis c.) Blood agar spreading flat pigmented, sometime with Beta- hemolysis, mucoid, confluent growth.

  18. Laboratory diagnosis 4-Biochemical tests: Oxidase test, catalase test all are positive. Catalase +ve Oxidase +ve

  19. Laboratory diagnosis 5- Glucose sugar fermentation acid but no gas. 6-Motility test: positive  inverted Christmas tree. 7-Antibiotic sensitivity: very important as this bacteria is multidrug resistance.

  20. Thank you

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