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Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion. Natalia C. Rodrigues, PhD; Lívia Assis, PhD; Kelly R. Fernandes, MSc; Angela Magri; Daniel A. Ribeiro, PhD; Roberta Brunelli, MSc; Daniela C. C. Abreu, PhD; Ana Claudia M. Renno, PhD. Aim

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Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

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  1. Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion Natalia C. Rodrigues, PhD; Lívia Assis, PhD; Kelly R. Fernandes, MSc; Angela Magri; Daniel A. Ribeiro, PhD; Roberta Brunelli, MSc; Daniela C. C. Abreu, PhD; Ana Claudia M. Renno, PhD

  2. Aim • Evaluate effects of 660 nm low-level laser therapy (LLLT) on muscle regeneration after cryolesion in rat tibiales anterior (TA). • Relevance • Studies have shown that laser irradiation favors regeneration of skeletal muscle, shortening inflammatory phase and accelerating proliferative and maturation phases of skeletal muscle regeneration.

  3. Method • 63 Wistar rats divided into: • Control group (CG). • 10 J/cm2 laser-treated group (G10). • 50 J/cm2 laser treated group (G50). • Compared 10 and 50 J/cm2 fluences 7, 14, and 21 d after cryolesion on TA through morphology and muscle tissue structure analysis. • Inflammatory process, granulation tissue, tissue structure, number of blood vessels, and immunomarkers were evaluated histologically and by immunohistochemistry analysis.

  4. Results • Main finding: • Both laser-treated groups presented better histological pattern. • Earlier recruitment of cell infiltrate • Higher amount of newly formed muscle fiber. • More organized muscle tissue structure at the lesion.

  5. Conclusion • Results suggest that LLLT, at both fluences, positively affects injured skeletal muscle in rats, accelerating muscle-regeneration process.

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