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VB17-specific forward. I R S S. CCAAAAGAACCCGACAGCTTT C TATCTCTGTGCCA GTAGTATTAGGAGCTCC TA CGAGCAGTACTTCGGGCCG. Cy5-JB2.7-specific reverse. RT-PCR. Cy5-IRSS PCR Product. mix and hybridize. Cy3-synthetic target mix.
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VB17-specific forward I R S S CCAAAAGAACCCGACAGCTTTCTATCTCTGTGCCAGTAGTATTAGGAGCTCCTACGAGCAGTACTTCGGGCCG Cy5-JB2.7-specific reverse RT-PCR Cy5-IRSS PCR Product mix and hybridize Cy3-synthetic target mix 5’-GAAGAGTATGGTTTCGTATACGT AAAAAAAAAAAAAAAAAAAAAAA GTAGTATTAGGAGCTCC-3’ (Arabidopsis thaliana LTP4) (poly-A) CDR3β IRSS clonotype 1 probe