1 / 12

INTRODUCTION EWEN SWB & PUSZTAI A, 1999 Effects of diets containing genetically modified

INTRODUCTION EWEN SWB & PUSZTAI A, 1999 Effects of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine, Lancet 354:1353-1355 BIOEMIT-SEMINAR 11-05-2004. Outline of experiment:. Diets (feeding this diet for 10 days)

toyah
Download Presentation

INTRODUCTION EWEN SWB & PUSZTAI A, 1999 Effects of diets containing genetically modified

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. INTRODUCTION EWEN SWB & PUSZTAI A, 1999 Effects of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine, Lancet 354:1353-1355 BIOEMIT-SEMINAR 11-05-2004 Plant Genetics Group

  2. Outline of experiment: Diets (feeding this diet for 10 days) Parent: Potatoes. No gene modification. No added lectin Parent +GNA: Potatoes+ added GNA lectin (25.4 ug/g) GNA-GM: Gene modified potato expressing 25.4ug/g GNA lectin GNA= Galanthus nivalis agglutinin (a lectin) Animals: Rats of ”similar weight” and age? Data: 6 rats, 5 samples from each (stomach, jejunum, ileum, caecum amd colon) Mean value of 6 rats given +/- SD Plant Genetics Group

  3. Ma etal, 2003 NatRevGen4:794 Plant Genetics Group

  4. Lectin expression: Transgenic potato made by expression of a snow drop lectin (binds to alfa-1-3-mannose residues). Fairly high level of lectin expression: 25.4 ug/g. Lectin containing edible plants normally have lectin levels of 0-10 ug/g. Some plants have lectin levels >5 mg/g Plant Genetics Group

  5. Digestive system: Stomach/thin intestinal: Produces 17x109 cells/24h 2-3 l HCl/day 1.5 l bukspytt/day 0.5 l galle/day 1.5 l tarmsaft/day Colon: 1 kg bacteria (+400 species) Absorbs 1.5 l ”water”/day Plant Genetics Group

  6. Overview Digistive system Samples from: Stomach Jejunum Ileum Caecum Colon Plant Genetics Group

  7. Duodenum Plant Genetics Group

  8. Jejunum & ileum Plant Genetics Group

  9. Results: Significant differences in crypt length observed for: Raw potatoes: Stomach: Parent vs Parent +GNA p= 0.03 Jejunum: Parent+GNA vs GNA-GM p=0.01 Caecum: Parent vs Parent +GNA p=0.02 Colon: All significant different Boiled potatoes: Caecum: Parent+GNA vs GNA-GM p= 0.04 Colon: All significant different Plant Genetics Group

  10. Ewen & Pusztai’s resultater Plant Genetics Group

  11. Plant Genetics Group

  12. Our body is prepared for digestion of plant food Plant Genetics Group

More Related