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Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and Karyotyping of parental, vector-infected and TG2-transfected LNCaP cells (B). B. A. Relative cell growth over 3 days. LNCaP LNCaP -EV LNCaP -TG2.

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Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

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  1. Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and Karyotyping of parental, vector-infected and TG2-transfected LNCaP cells (B) B A Relative cell growth over 3 days LNCaPLNCaP-EV LNCaP-TG2

  2. Fig. S2. Co-localization of TG2 and NF-κB in nuclear fraction of prostate cancer cells (A). Transient down regulation of NF-kB partially restored AR expression in PC3 cells (B). Putative NF-κB binding sites in the promoter of AR gene (C and D). p65 siR A B ContsiR p65 AR Actin LNCaP TG2 LNCaP Cont. LNCaP EV PC3 C Exon 1 -38 bp GACCCGACTCGCAAACTGTTGCATTTGCTCTCCACCTCCCAGCGCCCCCTCCGAGATCCCGGGGAGCCAGCTTG CTGGGAGAGCGGGACGGTCCGGAGCAAGCCCAGAGGCAGAGGAGGCGACAGAGGGAAAAAGGGCCGAGCTAG CCGCTCCAGTGCTGTACAGGAGCCGAAGGGACGCACCACGCCAGCCCCAGCCCGGCTCCAGCGACAGCCAACG CCTCTTGCAGCGCGGCGGCTTCGA AGCCGCCGCCCGGAGCTGCCCTTTCCTCTTCGGTGAAGTTTTTAAAAGCTG CTAAAGACTCGG +246 bp C N C N C N C N TG2 p65 Histones D • -760 bp • GGGTGATTTTGCCTTTGAGAGTCTGGATGAGAAATGCATGGTTAAAGGCAATTCCAGACAGGAAGAAAGGCAGAGA • AGAGGGTAGAAATGACCTCTGATTCTTGGGGCTGAGGGTTCCTAGAGCAAATGGCACAATGCCACGAGGCCCGATC • TATCCCTATGACGGAATCTAAGGTTTCAGCAAGTATCTGCTGGCTTGGTCATG -460 bp IκBα

  3. Fig. S3. TG2 and AR protein expression in selected prostate tumor samples (A). TG2 expression Is associated with increased accumulation of HIF-1α protein in prostate cancer cells (B) TG2 AR A B LnCap-TG2 LnCap-EV PC3  HIF-1α  TG2  β-actin

  4. Table S1 – Primers used to amplify various transcripts Gene designationPrimer Sequence (5’-3’) AR F: TTGGATGGCTCCAAATCAC R: GCAATGATACGATCG AGTTCC E-cadherin F: TCCCATCAGCTGCCCAGA A A R:TGACTCCTGTGTTCCTGT TA N-cadherin F: ACAGTGGCCACCTACAAAGG R: CCGAGATGGGGTTGATAATG VimentinF: GAG AACTTTGCCGTTGAA GC R: GCTTCCTGTAGGTGGCAATC FibronectinF: CAGTGGGAGACCTCGAGAAG R:TCCCTCGGAACATCAGAAAC ZEB1 F: CTGAAGAGGACCAGAGGCAG R: CCCAGAACTGCGTCACATGTC ZEB2 F: AAGTACCGCCACGAGAAGAA R: TTGTTTCTCATTCGGCCATT Snail F: TTCTTCTGCGCTACTGCTGCG R: GGGCAGGTATGGAGAGGAAGA TG2 F: TATGGCCAGTGCTGGGTCTTCGCC R: GGCTCCAGGGTTAGGTTGAGCAGG GAPDH F: ACCACAGTCCATGCCATCAC R: TCCACCACCCTGTTGCTGTA

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