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Supplementary file 8. Detection of expression of EpCOL1 to EpCOL12 in different tissue
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Supplementary file 8. Detection of expression of EpCOL1 to EpCOL12 in different tissue Total RNA isolated from several different tissues, including floral bud/opened flower (F), peduncles (P), capsules (C), leaves (L) and roots (R) were used as templates for semi-quantitative RT-PCR. This experiment was repeated four times with similar results. Loading and RNA quality control were verified by amplification of the constitutive E. pusilla Actin. F P C L R