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This article explores the different structures found in bacteria, such as capsules, flagella, pili, and cell walls. It discusses their functions, medical significance, and the role of the cell membrane in transport and energy production.
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Capsule or Glycocalyx • Outermost layer • Polysaccharide or polypeptide • Allows cells to adhere to a surface • Contributes to bacterial virulence-avoid phagocytosis
Flagella - motility E. coli O157:H7 Rotate like a propeller Proton motive force used for energy Presence/arrangement can be used as an identifying marker
Flagella - motility Rotate like a propeller Proton motive force used for energy Presence/arrangement can be used as an identifying marker • Peritrichous • Polar • Other (ex. tuft on both ends)
Cell movement is due to a series of “runs” and “tumbles” Flagella - motility Chemotaxis - Directed movement towards/away from a chemical • “Runs” are longer when cell is going in the right direction
Different types of pili Common pili (fimbriae) Sex pili - Conjugation
Cell Wall Provides rigidity to the cell (prevents it from bursting)
Cell Wall Provides rigidity to the cell (prevents it from bursting)
Cell Wall • Peptidoglycan - rigid molecule; unique to bacteria • Alternating subunits of NAG and NAM form glycan chains • Glycan chains are connected to each other via peptide chains on NAM molecules
Cell Wall • Peptidoglycan - rigid molecule; unique • to bacteria • Alternating subunits of NAG and NAM form glycan chains • Glycan chains are connected to each • other via peptide chains on NAM molecules
Cell Wall • Peptidoglycan - rigid molecule; unique to bacteria • Alternating subunits of NAG and NAM form glycan chains • Glycan chains are connected to each other via peptide chains on NAM molecules • Medical significance of peptidoglycan • Target for selective toxicity; synthesis is targeted by certain antimicrobial medications (penicillins, cephalosporins) • Recognized by innate immune system • Target of lysozyme (in egg whites, tears)
Cell Wall Gram-positive Thick layer of peptidoglycan Teichoic acids
Cell WallGram-negative • Thin layer of peptidoglycan • Outer membrane - additional membrane barrier; porins permit passage • lipopolysaccharide (LPS)
Cell WallGram-negative • Thin layer of peptidoglycan • Outer membrane - additional membrane barrier; porins permit passage • lipopolysaccharide (LPS) - ex. E. coli O157:H7 endotoxin - recognized by innate immune system
Cell WallGram-negative • Thin layer of peptidoglycan • Outer membrane - additional membrane barrier; porins permit passage • lipopolysaccharide (LPS) periplasm
Cytoplasmic membrane • Defines the boundary of the cell • Semi-permeable; excludes all but water, gases, and some small hydrophobic molecules • Transport proteins function as selective gates (selectively permeable) • Control entrance/expulsion of antimicrobial drugs • Receptors provide a sensor system • Phospholipid bilayer, embedded with proteins
Cytoplasmic membrane • Defines the boundary of the cell • Semi-permeable; excludes all but water, gases, and some small hydrophobic molecules • Transport proteins function as selective gates (selectively permeable) • Control entrance/expulsion of antimicrobial drugs • Receptors provide a sensor system • Phospholipid bilayer, embedded with proteins
Cytoplasmic membrane • Defines the boundary of the cell • Semi-permeable; excludes all but water, gases, and some small hydrophobic molecules • Transport proteins function as selective gates (selectively permeable) • Control entrance/expulsion of antimicrobial drugs • Receptors provide a sensor system • Phospholipid bilayer, embedded with proteins
Cytoplasmic membrane • Defines the boundary of the cell • Semi-permeable; excludes all but water, gases, and some small hydrophobic molecules • Transport proteins function as selective gates (selectively permeable) • Control entrance/expulsion of antimicrobial drugs • Receptors provide a sensor system • Phospholipid bilayer, embedded with proteins • Fluid mosaic model
Cytoplasmic membrane Electron transport chain Electron transport chain - Series of proteins that eject protons from the cell, creating an electrochemical gradient • Proton motive force is used to fuel: • Synthesis of ATP (the cell’s energy currency) • Rotation of flagella (motility) • One form of transport
If a function of the cell membrane is transport….. • How is material transported in/out of the cell? • Passive transport • No ATP • Along concentration gradient • Active transport • Requires ATP • Against concentration gradient
Types of transport • Passive transport • Simple diffusion • Facilitated diffusion • Osmosis • Active transport • System that uses proton motive force • System that uses ATP • Group translocation