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On-Demand Generation of Aqueous Two-Phase Microdroplets with Reversible Phase Transitions

CNMS Staff Science Highlight. On-Demand Generation of Aqueous Two-Phase Microdroplets with Reversible Phase Transitions. Scientific Achievement

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On-Demand Generation of Aqueous Two-Phase Microdroplets with Reversible Phase Transitions

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  1. CNMS Staff Science Highlight On-Demand Generation of Aqueous Two-Phase Microdroplets with Reversible Phase Transitions • Scientific Achievement • Aqueous two-phase microdroplets were generated on-demand within an oil microchannel, and reversible phase transitions between single-phase, two-phase, and core-shell microbeadstates were obtained via evaporation-induced dehydration and on-demand water rehydration. • Significance • The small femtolitervolume, static positioning, and reversible single-phase and two-phase states of our microdroplets provides an ideal system for future studies of the dynamic microcompartmentationand affinity partitioning of biomaterials. Furthermore, the reversible transitions between aqueous two-phase and solidified core-shell states could potentially be used for the controlled delivery of biomaterials. • Research Details • Aqueous solutions containing different polymers can be immiscible when mixed together above critical concentrations. Exploiting this, single-phase or two-phase microdroplets can be generated on demand as functions of polymer composition. • Evaporation of water from an initially single-phase microdroplet resulted in phase separation within the droplet due to increasing polymer concentration. • These phase changes were made reversible by rehydration via fusion of the microdroplet with another droplet of pure water. #5 1.4 nm (a) A single-phase droplet of 1% PEG and 1% dextran experienced a series of evaporation-induced phase transitions to: (b) initial phase nucleation, (c) complete aqueous two phase separation, and (d) core-shell microbead. J.B. Boreyko, P. Mruetusatorn, S.T. Retterer and, C.P. Collier, Lab Chip. DOI:10.1039/c3lc41122b (2013)

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