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UTICAJ 1-METILCIKLOPROPENA (1-MCP) KAO TRETMAN PREDČUVANJA NA PROCES ZRENJA KOD PLODOVA PARADAJZA RAZLIČITE ZRELOSTI. Zoran ILIĆ., Avital BEN-YOSEF., Yaccov PARTZELAN., Sharon ALKALAI-TUVIA and Elazar FALLIK
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UTICAJ 1-METILCIKLOPROPENA (1-MCP) KAO TRETMAN PREDČUVANJA NAPROCES ZRENJA KOD PLODOVA PARADAJZA RAZLIČITE ZRELOSTI Zoran ILIĆ., Avital BEN-YOSEF., Yaccov PARTZELAN., Sharon ALKALAI-TUVIA and Elazar FALLIK Faculty of Agriculture, Zubin Potok., Kosovska Mitrovica, SerbiaARO- Тhe Volcani Center, Postharvest Science of Fresh Produce,
1-MCP (1-methyilcyclopropene) as plant growth regulators : make advances in understanding the role of ethylene in plats. Role : Effects on ethylene inhibition. Non-toxic mode of action, negligble residue and active in very low concentrations. At standard temperature and pressure, 1-MCP is a gas with a molecular weight of 54 and a formula of C4H6. Effective concentrations from 2,5nl L-1 to 1µl L-1 mostly 300-900 ppb (nl L-1) Applied Temperature : 1-MCP is most commonly applied at 20-250C Time of duration : Treatment of 12-24 h were sufficient to achieve a full respons. Commertional application of 1-MCP to edible crops Under the trade name for edible products SmartFreshSM and for ornamentals EthylBlocSM 1-MCP was power that releases when mixed with water Method : Fumigant application, liquid spray application Place : Sealed container with plant material
A variety of factors may need to be considered when using 1-MCP including: cultivar, developmental stage, time from harvest to treatment and multiple applications Treated 1-MCP may have a variety of effects : on respirations, ethylene production, volatile production, chlorofil degradation and other color changes, protein and membrane changes, softening, disorders and diseases, chilling injury, acidity and sugars, antioxidant activity etc. Food use registration for 1-MCP has been obtained in : Argentina, Australia, Austriji, Brazil, Canada, Chile, Kosta Rica, Guatemala i Honduras, Israel, Mexico, Netherlands, New Zealand, South Africa, UK i USA. Registration crops, which are specific to counties , include : apple, apricot, avokado, kiwifruit, mango, melon, nectarine, peach, pear, pepper, persimon, pineapple, plum, squash, tomato and tulip bulbs.
Povrće, voće i ukrasno bilje kod kojih se primenjuju tretmani 1-MCP (Watkins and Miller., 2005)
Paradajz cv.1402 iz plasteničke proizvodje (centralni dela Izraela) je ubiran u različitoj fazi zrelosti Mature green zelen namenjen dozrevanju MG, Light pink svetlo ružičast i Light red svetlo crven plod.
Odabrani i očišćeni plodovi izloženi su u komorama dejstvu 1-MCP tretmana (300ppb), tokom 24časa na sobnoj temperaturi (20ºC). 1-MCP tretman je ostvaren kroz komercijalno sredstvo SmarthFreshTM (0,14% aktivne materije) rastvoren u vodi. Tretirani i kontrolni plodovi čuvani su na različitim temperaturama na 12ºC i 5ºC tokom 2 nedelje +4 dana na 20ºC (simulacija marketa).
Određivanje boje u osnovi znači odbijanje svetlosti od površine ploda a detektuje se svetloreflesionim metrom (Minolta kolorimetar kao hue angleº), pri čemu vrednost nivoa obojenosti od 30-40 predstavlja zreo plod, 60-80 ružičast i preko 90 zelen za dozrevanje. Na kraju čuvanja određeni su parametri kvaliteta: sadržaj suve materije (refraktometrijski %), sadržaj karotenoida određivan je spektrofotometrijski prateći metodu (Vinokura i sar., 2006) a čvrstoća ploda nedestruktivnom metodom pomoću penetrometra (Jamaica type-02, MFG.CO, New Jork) pri čemu je plod mekan <35, čvrst 35-40N i vrlo čvrst preko 40N.
Ukupna antioksidantna aktivnost (TAA) izražava se TEAC (trolox equivalent antioksidant capacity). Ovom metodom se određuju i hidrohilni (vitamin C) i lipofilni sadržaj (karotenoidi i vitamin E) zasnovani na ukupnom kapacitetu čišćenja radikala, i sposobnosti uklanjanja postojanog ABTS+ radikala (ABTS+) po metodi Vinokura i Rodova (2006).
Praćeno je i zdravstveno stanje, odnosno registrovano je prisustvo bolesti (% zaraženih plodova Botrytisom, Alternariom i Rhizopusom). Alternaria Botrytis Antracnosa Chilling injury Određivanje ukusa- arome Istraživanja su obavljena u department of Postharvest Science and Fresh Produce, Volcani Center u Bet Daganu, Izrael.
Effect of 1-MCP on diferent stage of tomato maturity after 2 week storage on 5ºC and 12ºC + 4days on 20ºC
Mature green – zelen za dozrevanje 2 week on 5ºC and 12ºC (control or 1-MCP) + 4days on 20ºC
Light pink – svetlo ružičast 2 week on 5ºC and 12ºC (control or 1-MCP) +4days on 20ºC
Light red – svetlo crveni2 week on 5ºC and 12ºC (control or 1-MCP) + 4days on 20ºC
Ukupna antioksidativna aktivnost - Total antioxidant activity (TAA) µmol TE/g fresh weigh
Uticaj temperature čuvanja i primene 1-MCP na razvoj obojenosti plodova paradajza različite zrelosti Influence of stogare temperatures and 1-MCP on color development on different stagesof tomato maturity
Content of carotenoides µg/g D.M. Sadržaj karotenoida mg/kg S.M.
Quality atributes of tomato fruit after two weeks storage on 5ºC i 12ºC + 4 days on 20ºC -shelf life Odlike kvaliteta plodova paradajza nakon 2 nedelje čuvanja na 5ºC i 12ºC + 4 dana na 20ºC( shelf-life) * Decay - Bolesti ( Alteraria i Botrytis)