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The proline accumulation in salt tolerant plants

The proline accumulation in salt tolerant plants. Elferra Swart, Maarten Weerenbeck en David van Diepen. Proline. Amino acid + compatible osmolyte Remain unchanged, H 2 O-soluble, no effect on other molecules First plants response water stress:

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The proline accumulation in salt tolerant plants

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  1. The proline accumulation in salt tolerant plants Elferra Swart, Maarten Weerenbeck en David van Diepen

  2. Proline • Amino acid + compatible osmolyte • Remain unchanged, H2O-soluble, no effect on other molecules • First plants response water stress: • decrease growth rate and turgor + prevention of feedback inhibition proline synthesis. (Crawford, 1989) • protects membranes and proteins against dangers of dehydration • Accumulation in cytosol leaves in salt tollerant plants, but also other plants during salt stress..

  3. Hypothesis • Proline accumulates when salt concentration becomes higher

  4. Method • Proline can be made visible by reaction with ninhydrine ( purple complex) • Extinction muesurement, ED 520 nm • Making standard line with known concentration • Calculation proline amount

  5. Results

  6. Results

  7. Results

  8. Results

  9. Discussion • No standard measures, but clear accumulation • In every plant, proline accumulates, what’s the difference? Tollerant plants don’t begin at 0! • Excellent results! • Hypothese correct without doubt • Further questions: • Does proline keeps rising? • What kind of trendline between salt and proline? • What is the correlation with other data?

  10. Conclusion • This was a very interesting talk • You can all applaud for me • If you really want, I can do the talk again Thank you!

  11. Credits: • Deze powerpoint is gemaakt door: • David van Diepen • Kijk voor meer powerpoints op: • www.davidvandiepen.nl Ik vind het leuk als u een berichtje achterlaat op mijn gastenboek!

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