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Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards

Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards. Eggs (5 days to 1 year). Larva (~10 days). Adult (1 week-2 months). Pupa (1-2 days). Vectors of human disease Infected blood meal Pathogens ingested Pathogens reproduce Next blood meal

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Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards

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  1. Female Aedes aegypti taking a blood meal on the arm of Dr. Edwards

  2. Eggs (5 days to 1 year) Larva (~10 days) Adult (1 week-2 months) Pupa (1-2 days)

  3. Vectors of human disease Infected blood meal Pathogens ingested Pathogens reproduce Next blood meal Pathogens transmitted through saliva Pathogens Ingested Pathogens Injected

  4. Mosquitoes enjoying an artificial blood meal

  5. Cow blood Parafilm membrane Feed mosquitoes

  6. Esophagus : No carboxypeptidae gene expression Cow blood Carboxypeptidase gene expression in the midgut cells

  7. Why did we think we had cloned a real; Carboxypeptidase A gene?

  8. Fluorescent Anopheles gambiae mosquito larvae

  9. Control Whole-mount in situ hybridization

  10. 15a-1: Eggshell Gene Vitelline envelope Vitelline envelope Follicular Epithelium ~ 10hr PBM

  11. Anti-LaCrosse Protein Human Anti-Viral Gene Induced by IFN-a/b Inhibits virus transcriptase Other antiviral activities? MxA

  12. Micro-inject embryos with DNA constructs

  13. SYBR green stained gel of mosquito genes expressed after a blood meal. PCR by Priscilla Mc. Dowell ‘01

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