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An in situ Sensor of Phytoplankton Community Structure Based on Light Absorption

An in situ Sensor of Phytoplankton Community Structure Based on Light Absorption. Gary Kirkpatrick, David Millie, Steven Lohrenz, Mark Moline, Ian Robbins and Oscar Schofield. Acknowledgements. National Science Foundation Biological Sciences Directorate Ocean Sciences Division

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An in situ Sensor of Phytoplankton Community Structure Based on Light Absorption

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  1. An in situ Sensor of Phytoplankton Community Structure Based on Light Absorption Gary Kirkpatrick, David Millie, Steven Lohrenz, Mark Moline, Ian Robbins and Oscar Schofield

  2. Acknowledgements • National Science Foundation • Biological Sciences Directorate • Ocean Sciences Division • National Oceanic and Atmospheric Administration • ECOHAB • MERHAB • Sea Grant • Florida Fish and Wildlife Conservation Commission • Florida Fish and Wildlife Research Institute • Office of Naval Research

  3. Finding, Tracking and Mapping Harmful Algae

  4. Our Approach to HAB Detection, Tracking and Mapping • Based on in situ particulate absorption spectra • Originally, analysis by similarity of 4th derivative spectra • Most recently, multiple species regression analysis (after Stæhr and Cullen)

  5. Advantages Over (traditional) Microscopic Enumeration • Readily automated – minimal human involvement • Good spatial and temporal coverage • Less weather dependent • Economical

  6. This Presentation • Single-species Similarity • laboratory to autonomous field application • Multiple-species Community Composition • laboratory to field trials

  7. FO FO LWCC Pump Valve Spectrometer Light Source Valve Valve Reference Water Cleaning Solutions Cross-flow Filter Optical Phytoplankton Discriminator (OPD)

  8. phcobilin Chl b Chl c Laboratory Class Comparisons

  9. Shipboard Underway HAB Detection

  10. OPD Map of HAB

  11. OPD-equipped Vehicles BreveBuster Payload REMUS – Propeller Driven Glider – Buoyancy Driven BSOP Vertical Profiler

  12. OPD-equipped Glider Surveying HAB Before Dive Gliding Launch Resulting Red Tide Distribution Mission Track & Satellite Image

  13. Glider Mission - Sep 28, 2004 – Oct 7, 2004

  14. Karenia brevis Similarity Contours

  15. Fitting Multiple Classes Least squares, multiple regression analyses (after Stæhr and Cullen, 2003). Optimization/reduction algorithm to minimize computational load.

  16. Laboratory Species MixesOPD vs CHEMTAX

  17. Multiple Class Library

  18. Library Standard Spectra Absorbance Fourth Derivative

  19. Natural Community ClassesOPD Chl avs CHEMTAX Chl a

  20. Discussion • Initial results encouraging. • Library of ‘standard’ species incomplete! • Libraries need to be regional. • Culture ID and condition are critical!! • Garbage in, garbage out!

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