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Introduction to the BioMart API. BioMart APIs. Biomart_plib - Objected Oriented Perl interface. Biomart_plib Architecture. Object Oriented Perl Based API to BioMart Datasets Uses XML configuration shared by all BioMart Software. Query logic. Configuration logic.
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BioMart APIs • Biomart_plib - Objected Oriented Perl interface
Biomart_plibArchitecture • Object Oriented Perl Based API to BioMart Datasets • Uses XML configuration shared by all BioMart Software
my $confFile = "/home/user/martRegistryFile"; my $initializer = BioMart::Initializer->new(‘registryFile’=>$confFile); my $registry = $initializer->getRegistry; Optional Initializer parameters: ‘action’ => ‘clean’ - replace the dataset configurations stored on the local file-system with those from the database and build a new, clean registry object ‘action’ => ‘update’ - replace any file-system dataset configurations modified since the last retrieval with the database copies and build a new registry object Default behaviour with no action specified is to generate the registry object using the cached file-system configurations if they exist, otherwise retrieve them from the database. Initializing API script
Optional Initializer parameters (cont) ‘mode’ => ‘lazyload’ - only keep a certain number of dataset configurations in memory at once for low memory machines and future scalability Default behaviour with no mode specified is to keep all configurations in memory. Initializing API script
my $query = BioMart::Query->new(‘registry’ => $registry ‘virtualSchemaName’ => ‘default’); $query->addAttribute('hsapiens_gene_ensembl','ensembl_gene_id'); or with optional virtualSchema and interface settings: $query->addAttribute('hsapiens_gene_ensembl','ensembl_gene_id’, ’default’,’default’); $query->addFilter('hsapiens_gene_ensembl','chromosome_name',['1']); $query->addFilter('hsapiens_gene_ensembl','hgnc_symbol',['FGFR1','IL2','DERL3']); Building Query
my $query_runner = BioMart::QueryRunner->new(); $query_runner->execute($query); $query_runner->printResults; Executing query and printing results
Print formatted header: $query_runner->printHeader; Print just first 20 results: $query_runner->printResults(20); Change the formatter from tab-separated default before execute the query: $query->formatter(‘FASTA’); The formatter has to have a corresponding module in lib/BioMart/Formatter implementing the FormatterI.pm interface (eg) CSV, TXT, GTF, XLS etc Executing query and printing results
Multi dataset queries my $query = BioMart::Query->new('registry'=>$registry, 'virtualSchemaName'=>'default'); $query->addAttribute('hsapiens_gene_ensembl','ensembl_gene_id'); $query->addAttribute('hsapiens_gene_ensembl','ensembl_transcript_id'); $query->addAttribute('mmusculus_gene_ensembl','ensembl_gene_id'); $query->addAttribute('mmusculus_gene_ensembl','ensembl_transcript_id'); This is the equivalent of picking human as the main dataset in the web interface and mouse as the optional second dataset (ie) the human attributes appear first in the result table followed by the mouse attributes. Note that BioMart queries are currently restricted to two datasets maximum for performance reasons and query planning technical difficulties.
Web services type access • To support GRID projects such as Taverna and other third party users who want to federate mart data without leaving a port to the database server openly accessible.
Web services type access http://test.biomart.org/cgi-bin/martservice?query= <?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE Query> <Query virtualSchemaName = "defaultSchema"> <Dataset name = "hsapiens_gene_ensembl"> <Attribute name = ”ensembl_gene_id" /> <Attribute name = "chromosome_name" /> <ValueFilter name = "chromosome_name" value = "1"/> </Dataset> </Query>
Web services type access Change format from default tab-separated format: <Query virtualSchemaName = "defaultSchema” formatter = “CSV”> <Dataset name = "hsapiens_gene_ensembl"> <Attribute name = ”ensembl_gene_id" /> <Attribute name = "chromosome_name" /> <ValueFilter name = "chromosome_name" value = "1"/> </Dataset> </Query>
Web services type access Get count instead: <Query virtualSchemaName = "defaultSchema” count=“1”> <Dataset name = "hsapiens_gene_ensembl"> <Attribute name = ”ensembl_gene_id" /> <Attribute name = "chromosome_name" /> <ValueFilter name = "chromosome_name" value = "1"/> </Dataset> </Query>
Web services type access Multi-dataset query: <Query virtualSchemaName = "defaultSchema"> <Dataset name = "mmusculus_gene_ensembl"> <ValueFilter name = "chromosome_name" value = "1"/> </Dataset> <Dataset name = "hsapiens_gene_ensembl"> <Attribute name = ”ensembl_gene_id" /> <Attribute name = "chromosome_name" /> <ValueFilter name = "chromosome_name" value = "1"/> </Dataset> </Query>
Web services type access (1) Recover the registry file: http://test.biomart.org/cgi-bin/martservice?type=registry (2) Recover the datasets available for a mart: http://test.biomart.org/cgi-bin/martservice?type=datasets& virtualSchema=default&mart=ensembl (3) Recover the filters available for a dataset: http://test.biomart.org/cgi-bin/martservice?type=filters& virtualSchema=default&dataset=hsapiens_gene_ensembl (4) Recover the attributes available for a dataset: http://test.biomart.org/cgi-bin/martservice?type=attributes& virtualSchema=default&dataset=hsapiens_gene_ensembl
MartJ • Java Interface to Biomart Datasets • Uses XML configuration shared by all BioMart Software
import org.ensembl.mart.lib.config.RegistryDSConfigAdaptor; URL confURL = null; try { confURL = InputSourceUtil.getURLForString(“data/defaultMartRegistry.xml”); } catch (MalformedURLException e) { throw new ConfigurationException("Warning, could not load " + “data/defaultMartRegistry.xml” + " file\n"); } RegistryDSConfigAdaptor adaptor = new RegistryDSConfigAdaptor(confURL, false, false, false); RegistryDSConfigAdaptor
import org.ensembl.mart.lib.config.DatasetConfig; DatasetConfig config = adaptor.getDatasetConfigByDatasetInternalName( "hsapiens_gene_ensembl", "default" ); DatasetConfig
import org.ensembl.mart.lib.Query; Query query = new Query(); //query needs some information from the DatasetConfig query.setDataSource(config.getAdaptor().getDataSource()); query.setMainTables(config.getStarBases()); query.setPrimaryKeys(config.getPrimaryKeys()); Query
Import org.ensembl.mart.lib.config.AttributeDescription; import org.ensembl.mart.lib.FieldAttribute; AttributeDescription adesc = config.getAttributeDescriptionByInternalName("gene_stable_id"); query.addAttribute(new FieldAttribute( adesc.getField(), adesc.getTableConstraint(), adesc.getKey() ) ); FieldAttribute/AttributeDescription
There are three types of Filter that can be added to the query, both are created using the attributes of a FilterDescription A. BasicFilter B. BooleanFilter (but watch for the two boolean 'flavors') C. IDListFilter Filter/FilterDescription
import org.ensembl.mart.lib.config.FilterDescription; FilterDescription fdesc = config.getFilterDescriptionByInternalName(“chr_name”); FilterDescription
import org.ensembl.mart.lib.BasicFilter; //The config system actually masks alot of complexity //with regard to filters by requiring the internalName //again when calling the getXXX methods query.addFilter(new BasicFilter( fdesc.getField(name), fdesc.getTableConstraint(name), fdesc.getKey(name), "=", "22" ) ); BasicFilter
import org.ensembl.mart.lib.BooleanFilter; //note there are different types of BooleanFilter //"boolean" and "boolean_num" if (fdesc.getType(name).equals("boolean")) query.addFilter(new BooleanFilter( fdesc.getField(name), fdesc.getTableConstraint(name), fdesc.getKey(name), BooleanFilter.isNULL ) ); else //”boolean_num” query.addFilter(new BooleanFilter( fdesc.getField(name), fdesc.getTableConstraint(name), fdesc.getKey(name), BooleanFilter.isNotNULL_NUM ) ); BooleanFilter
import org.ensembl.mart.lib.IDListFilter; String[] ids = new String[] { “ENSG00000146556.4”, “ENSG00000197194.1”, “ENSG00000197490.1”, “ENSG00000177693.1” }; query.addFilter(new IDListFilter( fdesc.getField(name), fdesc.getTableConstraint(name), fdesc.getKey(name), ids ) ); IDListFilter
import org.ensembl.mart.lib.Engine; import org.ensembl.mart.lib.FormatSpec; Engine engine = new Engine(); engine.execute( query, new FormatSpec(FormatSpec.TABULATED, "\t"), System.out ); Engine
In the future, MartJ will be refactored to use the more flexible Architecture that we developed for the perl based software. Future of MartJ