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LYCOPENE EXTRACTION FROM TOMATOES

LYCOPENE EXTRACTION FROM TOMATOES. ANA ÁLVAREZ MARTÍN LAURA HERNANDEZ SALAMANQUES. CONTENT. Introduction Extraction using a solvent Supercritical extraction Extraction assisted by enzyme. LYCOPENE. Type of carotenoid Aditive , colorant and antioxidant Tomato pomace Ripe tomatoes.

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LYCOPENE EXTRACTION FROM TOMATOES

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  1. LYCOPENE EXTRACTION FROM TOMATOES ANA ÁLVAREZ MARTÍN LAURA HERNANDEZ SALAMANQUES

  2. CONTENT • Introduction • Extraction using a solvent • Supercritical extraction • Extraction assisted by enzyme

  3. LYCOPENE • Type of carotenoid • Aditive, colorant and antioxidant • Tomatopomace • Ripetomatoes

  4. CONTENT • Introduction • Extraction using a solvent • Supercritical extraction • Extraction assisted by enzyme

  5. EXTRACTION USING A SOLVENT • Fat-soluble: extractedwith organic solvents • Raw material: tomato waste skin • Solvent: hexane:acetone:alcohol (2:1:1) + BHT. • Variables

  6. EXTRACTION USING A SOLVENT VARIABLES Number of extraction Solvent/meal ratio Solvent/mealratio Particlesize

  7. EXTRACTION USING A SOLVENT VARIABLES Temperature Number of extractions Particlesize Number of extractions

  8. EXTRACTION USING A SOLVENT VARIABLES Extractiontime Number of extractions

  9. EXTRACTION USING A SOLVENT OPTIMUS CONDITIONS solvent/meal: 30:1 v/w 4 extractions 50 °C particle size 0.15 mm extraction time 8 min. Lycopene yield: 1.99 mg/100 g

  10. CONTENT • Introduction • Extraction using a solvent • Supercritical extraction • Extraction assisted by enzyme

  11. SUPERCRITICAL • Supercritical fluid extraction (SFE) • Tc, Pcsolvent • CO2: • low critical temperature, • not toxic, • not flammable, • low cost , • high purity

  12. SUPERCRITICAL • Semi-continuos process • Equipment: an extractor, a back pressure regulator, a chiller, a high pump for CO2, collection vials and a wet gas meter. • Optimization: tomato seed oil (co-solvent)

  13. SUPERCRITICAL Constantflowrate:

  14. SUPERCRITICAL Constantpressure:

  15. SUPERCRITICAL Constanttemperature:

  16. SUPERCRITICAL • OTHER FACTORS: • Risingmethod • Tomatopeeltoseed ratio • Particlesize • Degradationlycopene

  17. CONTENT • Introduction • Extraction using a solvent • Supercritical extraction • Extraction assisted by enzyme

  18. EXTRACTION ASSISTED BY ENZYME • Improvelycopeneextraction • Response surfacemethodology (RSM) • Chemicals: • Acetona, ethanol, hexane • BHT • Enzimes: • Peclyve PR • Cellulyve 50LC

  19. EXTRACTION ASSISTED BY ENZYME • Optimalconditions: • T=30ºC • Extraction time=3.18h • Enzyme load=0.16kg/kg • Enzymepreparation= 50:50 Pec-Cel

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