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What is a Fingerprint?. A Fingerprint is:. A deposit of: 98% perspiration 1% amino acids 1% oils Formed on the friction ridges of fingers, palms & feet These materials are left on the surface contacted. Major Components Na + K + Ca 2+ Fe 2+ Cl - F - Br - I -. HCO 3 - PO 4 3-
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A Fingerprint is: • A deposit of: • 98% perspiration • 1% amino acids • 1% oils • Formed on the friction ridges of fingers, palms & feet • These materials are left on the surface contacted
Major Components Na+ K+ Ca2+ Fe2+ Cl- F- Br- I- HCO3- PO43- SO42- NH4OH Inorganic Constituents of fingerprint residue from sweat
Inorganic Constituents of fingerprint residue from sweat Minor Components Mg2+ Zn2+ Cu2+ Co2+ Pb2+ Mn2+
Proteins Amino Acids Lipids Glucose Lactate Urea Pyruvate Creatine Creatinine Glycogen Uric Acid Vitamin Sterols Organic Constituents of fingerprint residue from sweat
Types of Fingerprints Left at the Crime Scene • Latent print impressions • Patent print impressions • Plastic print impressions • Katent print impressions
Patent Print • Readily visible to the naked eye • Usually the result of some substance coating the ridge detail and then allows the transfer transfer of ridge detail to a surface that is touched. • More common substances at a crime scene: • Blood, paint, grease • Photograph to preserve as evidence
Plastic Prints • Visible print to the naked eye where the print detail is usually in a soft surface • More common surfaces/substances seen at a crime scene: • Window putty, dust, dirt, mud • Photograph to preserve as evidence
Katent Prints • Prints made by pre-puberty children • Minutia more compact than adult • Not made by sweat remnants • Easy to lose on objects because mainly composed of water
Latent Prints • Prints not visible to the naked eye • Prints need to be made visible • through:powders, chemical processes, or lasers • Most common type of impressions left at crime scenes.
Non-destructive Methods of Lifting Prints • Visual exams • Alternate light source (ALS) • Photography
Destructive Methods of Lifting Prints • Physical Means (better for non-porous): • Conventional Powders, Magnetic Powders, Fluorescent Powders • Chemical Means (better for porous materials): • Ninhydrin, Silver nitrate, Superglue (Cyanoacrylate ester), Iodine fuming, Physical Developer (PD), 1,8-diazafluorenone (DFO)
What to include on lift card • At a minimum include: • Case # • Date • Print #/letter • Initials of lifting technician
Practice lifting using conventional powder after being shown the technique using a slide
Practice lifting using magnetic powder after being shown the technique using a slide
Chemical Lifting Techniques Use with porous objects
Iodine • Not really a chemical reaction • Temporary • Place a few crystals in closed container with object. • Iodine sublimates • Photograph print to preserve it
AgNO3(aq) • Cover area with solution (we will use a brush) • Expose area to high energy (e.g. UV) light source (be careful not to overexpose print) • Photograph to help preserve • AgNO3(aq) reacts w/ salts that are present AgNO3(aq) + Cl- AgCl- + UV 2AgCl 2Ag0 +Cl20
Ninhydrin • Ninhydrin dissolved in alcohol (ethyl, isopropyl, methyl, or butyl alcohol) • Reacts w/ amino acids in print (forms Ruhemann Purple) • Place object in solution (or spray solution on large object, wear protection) to soak • Allow object to dry (warm & humid area best) • Iron object with steam setting (introduces warmth & humidity)
Ruhemann Purple Ninhydrin reaction
2 more photographic processes • 1-8, diazafluoren-9-one (DFO) • Physical Developer (PD) • Both react with salts that are left in the prints • Used more often than AgNO3(aq) but similar processes
Gentian violet (crystal violet) • Stains fats/oils left behind • To make solution- .02g/80mL dH2O • Used for finding prints on “sticky” side of tape • Place tape piece in solution • Rinse tape with water • Photograph prints that are present
Super Glue (Cyanoacrylic Esters) • Uses polymerization • Developed prints appear white • Heat process: • Place control print in developing area • Place 2-3 drops/gal of container on dish • Heat (we will use coffee warmer) • NaOH method: • Place control print in developing area • Place ~10 drops on cotton ball containing NaOH