10 likes | 159 Views
Attractant. Δ [CheY-P]. Photon. Δ [Ca 2+ ]. Ca 2+ Fluorescence. E. coli chemotaxis network. Output. Signal. Optimal Strategy of E. coli Chemotaxis Network from Information Processing View
E N D
Attractant Δ[CheY-P] Photon Δ[Ca2+] Ca2+ Fluorescence E. coli chemotaxis network Output Signal Optimal Strategy of E. coli Chemotaxis Network from Information Processing View Lin Wang and Sima SetayeshgarDepartment of Physics, Indiana University, Bloomington, Indiana 47405 Stimulus Signal Transduction Pathway [CheY-P] Motor Response Flagellar Bundling Motion Focus Model Validation Effect of Correlation Timeτ Motivation My first step is to investigate the effect of correlation time τto the I/O mutual information rate of the chemotaxis network. Chemical signaling cascade is the most fundamental information processing unit in biological systems. Generally, it converts external stimulus to change in concentration of intracellular signaling molecules. The preliminary result suggests that E. coli varies its response function under signals with different statistics. My goal is to understand how signal transduction pathways, such as the chemotaxis network, may adapt to the statistics of the fluctuating input so as to optimize the cell’s response. My direction is to construct a measurement of information transmission rate and investigate the role of varying response. Adaptation E. Coli Chemotaxis[3] Photoreceptor[1,2] Effect of τ in I/O relation Signals: μ=1 μM,σ2 = μ and τ = 0.1, 0.3, 0.8, 1 sec, respectively. At τ > 0.8 sec, the response does not change any more. (This holds for signals with different mean values) Experiment: Cell response when expose to a step change of aspartate from 0 to 0.1 mM, beginning at 5 sec[9]. Simulation: Cell response when exposed to a step change in aspartate from 0 to 10 μM, beginning at 5 sec. Numerical Implementation Response of drosophila photoreceptor to photon absorption. Response of E. coli to external attractant. Yellow: CheY-P relative level. Adaptation time Parameter values of chemotaxis network Use E. coli chemotaxis network as a prototype to explore the general information processing principle in biological systems. Table I: Signal Transduction Network Table II: Activation Probabilities Effect of τ in I/O mutual information [1] R. C. Hardie et al. (2001) Nature 413, 186-193 [2] J. Oberwinkler et al. (2000) PNAS 97, 8578-8583 Experiment: Transition time to step change of external attractant (aspartate, AIbu) and repellent (L-leucine)[10]. Simulation: Adaptation time to step change of concentration of aspartate. E. coli Chemotaxis The I/O mutual information rate of E. coli chemotaxis network is plotted as a function of correlation time τ. The Gaussian distributed signals used here have means of 1, 3, 5, and 10, respectively. Motor CCW and CW intervals Chemotaxis, motion toward desirable chemicals (usually nutrients) and away from harmful ones, is achieved through continuous ‘runs’ and ‘tumbles’. Table III: Initial Protein Levels Fluorescently labeled E. coli (Berg lab) Body size: 1 μm in length, 0.4 μm in radius Flagellum: 10 μm long, 45 nm in diameter Simulation: Distribution of wild-type E. coli motor CW (grey) and CCW (black) intervals. Effect of varying response Use found r(s1) under input signal μ1=1 μM,σ12 = μ1, τ1 = 1 sec to find P(r) for different input signals, and calculate the mutual information between r(s1) and sk. Experiment:Distribution of wild-type E. coli motor CW (grey) and CCW (black) intervals[11]. Chemotaxis network Comment on agreement: the simulation results are in good agreement with experiments, although, the adaptation differ by a factor of unit in time scale. Simulating reactions Reactions are simulated using Stochsim[5] package, a general platform for simulating reactions stochastically. Reactions have a probability p to occur. The calculated I/O mutual information rate of E. coli chemotaxis network maximizes under the condition that the response and the input signal matches. Numerical [9] S. M. Block et al. 1982 Cell 31 215-226 [10] H. C. Berg et al. 1975 PNAS 72 3235-3239 [11] T. Emonet et al. 2005 Bioinformatics 21 2714-2721 • Uni-molecular reaction • Symbols: • n: Number of molecules in reaction system • n0: Number of pseudo-molecules • NA: Avogadro constant • p: Probability for a reaction to happen • Δt: Simulation time step • V: Simulation volume Input-Output Relation The chemotaxis signal transduction pathway in E. coli – a network of ~50 interacting proteins – converts an external stimulus (change in concentration of chemo-attractant / repellent) into an internal stimulus (change in concentration of intracellular response regulator, CheY-P) which in turn interacts with the flagella motor to bias the cell’s motion. Utilizing this realistic and stochastic numerical implementation that is consistent with experiments, we explore E. coli chemotaxis network from the standpoint of general information-processing concepts. • Bi-molecular reaction From R. M. Berry, Encyclopedia of Life Sciences Physical constants for motion: Cell speed: 20-30 μm/sec Mean run time: 1 sec Mean tumble time: 0.1 sec Conclusions Motor response[6] The chemotaxis network is able to extract once the input signal varies slower relative to the response time of the chemotaxis network. Under an input signal with specific statistics, the chemotaxis network varies its response to optimize the cell’s response, maximizing the mutual information between input signal and output response. Motor response A simple threshold model is used to model motor response. The motor switches state whenever CheY-P trace (blue trace) crosses the threshold (red line) Input signal Artificially generated Gaussian distributed time series with correlation time τ. Output Number of CheY-P molecules is used as the system output. Adaptation [5] C. J. Morton-Firth et al. 1998 J. Theor. Biol.. 192 117-128 [6]T. Emonet et al. 2005 Bioinformatics 21 2714-2721 Adaptation is an important and generic property of signaling systems, where the response (e.g. running bias in chemotaxis) returns precisely to the pre-stimulus level while the stimulus persists. Adaptation functions from short time scale (impulse) to long time scale (evolution). It allows the system to compensate for the presence of continued stimulation and to be ready to respond to further stimuli. Future Work Mutual Information Use a realistic description of motor to Replace the simple threshold model of motor response. Taken into account the clustering effect among trans-membrane aspartate receptors to improve the performance of the numerical implementation. Role of adaptation time. The average information that observation of Y provide about the signal X, is I, the mutual information of X and Y[7]. I is at minimum, zero, when Y is independent of X, while it is at maximum when Y is completely determined by X. The I/O mutual information rate can be calculated by the following equation[8]. Adaptation variation[3] Adaptation[3] I/O relation under signals with different statistics. (τ = 1 sec) 1.Signal is binned. 2. response is the average of responses to signals falls within each bin. Upper: Gaussian distributed signal (μ=3 μM,σ2 = μ, τ = 1 sec) Lower panel: Response to the input signal. Run Bias s: Input signal; P(s): probability distribution of signal r: response; P(r): probability distribution of response r(s): I-O relation, mapping s to r. n: noise; P(n|r): noise distribution conditioned on response Adaptation to various step change of attractant serine (mM). Attractant: 30 μM aspartate. Repellent: 100 μM NiCl2 [7] Spikes, Fred Rieke et al. 1997, p122-123 [8] N. Brenner et al. (2000) Neuron. 26 695-702 [3] Sourjik et al. (2002) PNAS. 99 123-127 [4] H. C. Berg, (1975) PNAS. 72 71-713