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Liquid cultures to Lysis : Movie Clip from Teachers domain

Liquid cultures to Lysis : Movie Clip from Teachers domain. Student Guide Lab 6. What is made of DNA? What is made of protein? How is this related to rfp ?. What is made of DNA? What is made of protein?. AraC dimer. rfp. Rfp protein. arabinose. rfp. Bruce Wallace. RFP.

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Liquid cultures to Lysis : Movie Clip from Teachers domain

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  1. Liquid cultures to Lysis: Movie Clip from Teachers domain Student Guide Lab 6

  2. What is made of DNA? What is made of protein? How is this related to rfp?

  3. What is made of DNA? What is made of protein?

  4. AraCdimer rfp Rfp protein arabinose rfp

  5. Bruce Wallace RFP

  6. Growth of transformed bacteria on various plates Bruce Wallace P+ plates LB LB/amp LB/amp/ara P- plates No growth LB LB/amp

  7. The AraC Regulatory system

  8. Lab 5a: Questions • Pass cultures in the room • Expectations • Why they grew this way? PreLab 6 Questions - All about the AraCoperon

  9. araC gene PBAD rfp gene mRNA araC protein RFP expression Bruce Wallace Transcription Translation

  10. NO ARABINOSE: araC protein prevents RFP transcription by causing a loop to form in the region of the fp gene r araC gene PBAD rfp gene RFP expression Bruce Wallace araC protein

  11. With ARABINOSE: araC gene PBAD rfp gene RFP (red fluorescent protein) mRNA Transcription RFP expression Bruce Wallace Arabinose – araC protein complex prevents DNA looping and helps to align RNA polymerase on the promoter site (PBAD). arabinose Translation RNA polymerase arabinose – araC protein complex araC protein

  12. LB/amp/ara broth Preparing an overnight culture of E. Coli for RFP expression Bruce Wallace Colony isolation and culture

  13. Growth of transformed bacteria on various plates Bruce Wallace P+ plates LB LB/amp LB/amp/ara P- plates No growth LB LB/amp

  14. Purification of RFP from an overnight culture Bruce Wallace Overnight culture Cell pellet with RFP Lysed cells Pellet cell debris RFP with binding buffer

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