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Su Dong Kim 2 , Ki-Roo Shin 1 & Byoung-Tak Zhang 1,2*

Molecular Immnuocomputing with Application to Alphabetical Pattern Recognition Mimics the Characterization of ABO Blood Type. Su Dong Kim 2 , Ki-Roo Shin 1 & Byoung-Tak Zhang 1,2* 1 School of Computer Science & Engineering, Seoul National University, Seoul, Korea

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Su Dong Kim 2 , Ki-Roo Shin 1 & Byoung-Tak Zhang 1,2*

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  1. Molecular Immnuocomputing with Application to Alphabetical Pattern Recognition Mimics the Characterization of ABO Blood Type Su Dong Kim2 , Ki-Roo Shin1 & Byoung-Tak Zhang1,2* 1School of Computer Science & Engineering, Seoul National University, Seoul, Korea 2Interdisciplinary Program in Cognitive Science, Seoul National University, Seoul, Korea

  2. Introduction DNA computing vs. Peptide computing

  3. Four Types of Noncovalent Bonds in Peptide Computing

  4. Structure of Antibody with Its Antigen

  5. Molecular Immunocomputing Mimics the Characterization of ABO Blood Type

  6. The ABO Blood Grouping System

  7. The Structure of Terminal Sugars in the A, B, & O Blood Antigens (Gal; Galactose, GlcNAc; N-acetylglucosamine, Fuc; Fucose, and GalNAc; N-acetylgalactosamine)

  8. Nucleotide Sequences of ABO Alleles Identified by PCR-SSCP Analysis (Ogasawara et al., 1996)

  9. Methods Designing the peptide sequences Purifying the corresponding antibodies Preparation of protein microarray chips

  10. Four Kinds of DNA Code Operators

  11. Designing the Peptide Sequences “O”- DNA template sequence: 5’- atcc ctt tcg ggc ttc ggg ctt cgg gct tcg ggc ttc ggg ctt tcc ctt – 3’ “O”- RNA transcript sequence: 3’ – uguagg gaa agc ccg aag ccc gaa gcc cga agc ccg aag ccc gaa agg gaa – 5’ “O”- peptide sequence: HOOC - *C G K R A E P K P K P S R A E P K G K -NH2

  12. The Universal Genetic Code

  13. Hapten-Carrier Conjugation & Immunization

  14. Preparation of Protein Microarray Chips (Haab et al., 2001a) (Zhu & Snyder, 2003)

  15. Discussion (a) (c) (d) (b) Structures of the peptide CTP3 (VEVPGSQHIDSQKKA) (a), PS1 (HQLDPAFGANSTNPD) (b), SHaPrP (104-113 ; KPKTNMKHMA) (c), and peptide with antigen binding site (d). [(a), (b), and (c): drawed by the Swiss-PdbViewer (Guex & Peitsch, 1997); (d): adapted from Lescar et al., 1991.]

  16. Classes of Capture Molecules for Protein Microarrays Sandwich immunoassay (Templin et al., 2002)

  17. Conclusion

  18. Acknowledgments This research was supported in part by the Ministry of Education & Human Resources Development under the BK21-IT Program, The Ministry of Commerce, Industry and Energy through the MEC project, and the NRL Program from Korean Ministry of Science & Technology. The ICT at Seoul National University provided research facilities for this study.

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