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SERDP Perchlorate Research

Attachment 5. SERDP Perchlorate Research. Catherine Vogel SERDP/ESTCP Program Manager for Cleanup. SERDP-Funded Perchlorate Research. Research Focus:

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SERDP Perchlorate Research

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  1. Attachment 5 SERDP Perchlorate Research Catherine Vogel SERDP/ESTCP Program Manager for Cleanup

  2. SERDP-Funded Perchlorate Research • Research Focus: • Lab-, bench- and pilot field-scale research to develop biological approaches for the cost effective in-situ treatment of groundwater contaminated with ammonium perchlorate • Objectives • Improve fundamental understanding of the chemical, physical, and biological phenomena related to perchlorate reduction; • consider a wide range of sites and hydrogeochemical conditions; • provide information to predict practicability of proposed technologies under field conditions.

  3. In-Situ Bioreduction and Removal of Ammonium Perchlorate • Southern Illinois University; FY00 – FY02; 384 K • Technical Approach: • Identify & enumerate predominant perchlorate reducers in contaminated environments; • Identify genes that encode the chlorite dismutase enzyme; • Identify environmental controls on metabolic capabilities • Investigate techniques for in-situ stimulation • Develop a molecular probe specific for all perchlorate reducing bacteria. • Benefits: • Fundamental understanding of biological perchlorate reduction mechanisms

  4. In-Situ Bioremediation of Perchlorate • Envirogen; FY00 – FY01; $579K • Technical Approach. • Microcosm and column studies using aquifer material from • several perchlorate contaminated sites to: • Identify the most effective electron donor and effect • of competing electron acceptors • Evaluate environmental variables on perchlorate • reduction • Evaluate need for bioaugmentation • Develop models to evaluate substrate application • rates and perchlorate reduction kinetics • Benefits: • Collect information for design of pilot-scale remediation • effort

  5. In-Situ Bioremediation of Perchlorate-Impacted Groundwater • GeoSyntec Consultants; FY00 – FY 01; $355K • Technical Approach. • Confirm indigenous groundwater microorganisms can be • stimulated to biodegrade perchlorate. • Evaluate the ubiquity of the process at sites having • differing groundwater conditions. • Assess the suitability of the process for treating mixed • plumes using microcosm studies. • Conduct small field pilot-test at a perchlorate • contaminated site. • Benefits: • Demonstrate perchlorate biodegradation under field • conditions • Initial Design & Cost Data for potential technology scale- • up

  6. Envirogen S. Illinois Univ. GeoSyntec • Ubiquity of • perchlorate degraders • (microcosms) • Enrichment/Isolation • Identification • Enzymology • Molecular Probes • Biostimulation/ • Bioaugmentation • Effect of Env. Conditions • pH • temperature • salinity • ion concentration • alternate e- acceptors • co-contaminants • perchlorate concentration • Modeling/Preliminary Design (3-5 sites) (6 sites) (5 sites) pure culture + microcosms microcosms + columns microcosms + field pilot test pure + mixed culture mixed culture mixed culture low - med low high

  7. Perchlorate Sites Providing Samples • Southern Illinois University: • Samples collected thus far from Indian Head NSWC, MD; Jet Propulsion Laboratory, CA; and USN San Nicolas Island, CA. • Envirogen: • Jet Propulsion Lab, CA; Indian Head NSWC, MD (2 sites); Rocky Mt commercial site, CO; Oyster, Virginia (pristine site). Longhorn AAP, TX is a possible 5th site. • GeoSyntec: • Aerojet, Sacramento, CA; Edwards AFB, CA; USN Allegany Ballistics Lab, WV; American Pacific Corporation, NV; USN San Nicolas Island, CA; Boeing Alpha & Sigma Complex, CA.

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