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Raster Image Correlation Spectroscopy

Raster Image Correlation Spectroscopy. Seminario di relazione per la partecipazione a: 3rd European Workshop on Advanced Fluorescence Imaging and Dynamics (8-12 Ottobre 2012) Di Paolo Pozzi. FCS. Raster Imaging. I. t. How to do RICS. Spatial autocorrelation:. Fitting Function.

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Raster Image Correlation Spectroscopy

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  1. Raster Image Correlation Spectroscopy Seminario di relazione per la partecipazione a: 3rd European Workshop on Advanced Fluorescence Imaging and Dynamics (8-12 Ottobre 2012) Di Paolo Pozzi

  2. FCS

  3. Raster Imaging I t

  4. How to do RICS Spatial autocorrelation:

  5. Fitting Function Diffusive Component: Scanning Component:

  6. What could go wrong Scan too slow Undersampling Phototube shot noise is time correlated!!!

  7. What could go wrong Immobile population: RICS profile Uniform concentration and uniform diffusion coefficient are needed for rics!!!

  8. Immobile removal Subtracting averange value Obtaining negative value An arbitrary constant can be added, but the information is partially lost

  9. Number of images and image area 100 nM EGFP

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