1 / 24

Bacterial genetics

Bacterial genetics. Gram-positive bacteria transform DNA using a transformasome complex. Figure 9.1. Gene Transfer by Conjugation. Conjugation is the transfer of DNA from one bacterium to another, following cell-to-cell contact.

veda-cote
Download Presentation

Bacterial genetics

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Bacterial genetics

  2. Gram-positive bacteria transform DNA using a transformasome complex. Figure 9.1

  3. Gene Transfer by Conjugation Conjugation is the transfer of DNA from one bacterium to another, following cell-to-cell contact. - It is typically initiated by a special pilus protruding from the donor cell. Figure 9.2

  4. Figure 9.3

  5. Figure 9.4

  6. An integrated F-factor can excise from the chromosome. - Aberrant excision results in an F′ factor or F′ plasmid,which carries chromosomal genes. Figure 9.5

  7. Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR What medium should we use to obtain 1. leu+ transconjugants 2. pro+ transconjugants 3. lac+ transconjugants

  8. Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR 1. leu+ transconjugants 2. pro+ transconjugants 3. lac+ transconjugants What are the map positions of leu, pro, and lac?

  9. Generalized Transduction Figure 9.7

  10. Figure 9.9

  11. Specialized Transduction Figure 9.8

  12. Generalized Recombination Figure 9.14

  13. Figure 9.15

  14. Figure 9.30

  15. IS elements transfer by one of 2 mechanisms: - Replicative or nonreplicative transposition Mobile Genetic Elements Figure 9.31

More Related