ABSTRACT

1. ABSTRACT STANDARD PARAMETER DETERMINATION OF RAW MATERIAL AND ETHANOL EXTRACT 96 % TURMERIC RHIZOME (Curcuma domestica Val.) Dyah Handayani Turmeric (Curcuma domestica Val.) as medicinal plant found in almost Indonesia region, have been used as traditional medicine. Turmeric contains 3-5 % yellow pigment which is called curcuminoid. Curcuminoid in turmeric consist of curcumin, monodesmetoksicurcumin, bidesmetoksicurcumin. So far, in its uses, Turmeric will be developed as standarized herbal drug and phytopharmaca product. Phytopharmaca as pharmaceuticals product have to comply qualification of modern drug, that is standarized finished product and raw material. Standard parameter of raw material and extract which will be used, have to conducted before to get standarized product. The aim of this study is to determine spesific parameter and nonspesific parameter of raw material and extract turmeric rhizome. Turmeric rhizome which have been harvested, was washed, cuted, dried in open air and grinded into powders. In this stage, spesific parameters and nonspesific parameters determination of raw material are conducted. Rhizome powder made into extract by meceration in ethanol 96 %. The obtained extract are condensed by rotavapor and dried with 5 % mixture of Cab-O-Sil and Avicel 1:1. Standard parameter of extract are conducted. The result showed non-spesific parameters of raw material were total ash was (11,31 ± 0,08)%, acid insoluble ash was (6,89 ± 0,20)%, water soluble ash was (1,47 ± 0,16)%, water content was (10,21± 0,20)%, volatile oil content was (3,93 ± 0,23)% and loss on drying was (16,16 ± 0,28)%. The spesific parameters of raw material showed that water soluble extractive substance was (20,71± 0,51)%, alcohol soluble extractive substance was 13,49 ± 0.11)%, and curcuminoid content was (5,79 ± 0,51). The spesific parameters of raw material also examined macroscopic and microscopic of raw material. The result non-spesific parameters ethanol extract showed that loss on drying was (13,67 ± 0,50)%, Cu content was (1,479 ± 0.034) ppm, Zn content was (1,0335 ± 0,0410) ppm, )%, Pb content was (0,4499 ± 0,2158) ppm, positif in DDT (qualitatively), and negatif from microbial impurities. The spesific parameters of ethanol extract show that curcuminoid content was (23,77 ± 1,15)%. The spesific parameters of ethanol extract also determined by general appearance of dried extract. Keyword : Standard parameter, raw material, ethanol extract, turmeric, Curcuma domestica Val.