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MICROSCOPES. Microscopes reveal the world of the cell : Advances in science often coincide with advances in tools and technology Use of the microscope led to development of the cell theory: - All living things are made of cells
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Microscopes reveal the world of the cell: • Advances in science often coincide with advances in tools and technology • Use of the microscope led to development of the cell theory: - All living things are made of cells - cells come from other cells by reproduction.
Terms: Magnification: the increase in the apparent size of an object Resolution: a measure of the clarity of an image (The ability of an optical instrument to show two close objects as separate)
Types of microscopes: • Light microscope: works by passing visible lightthrough the specimen - Glass lenses bend light to magnify the image - Light microscopes can effectively magnify images only about 1,000 times
2. Electron microscope: uses a beam of electrons instead of light Advantage: much greater resolutionandmagnification Disadvantage: preparing the specimen kills the cells
There are 2 types of electron microscopes: Transmission electron microscope:(TEM) used to study details of internal cell structure • Specimens are cut into extremely thin sections and stained with atoms of heavy metals • A beam of electrons is aimed throughthe slice
Scanning electron microscope:(SEM) examines the cell surface after it has been coated with a thin layer of metal