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Microscopes. Development of the microscope. Anton van Leeuwenhoek Father of Microbiology Made his own lenses (some as powerful as the microscopes we use in class) First person to see living microorganisms. Microscopes. Compound light microscope. Focuses light
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Development of the microscope • Anton van Leeuwenhoek • Father of Microbiology • Made his own lenses (some as powerful as the microscopes we use in class) • First person to see living microorganisms
Compound light microscope • Focuses light • Shines light through objects • Magnifies up to 1000 times (ours are 400x) • Can view live organisms • Uses combination of lenses www.science.smith.edu/.../Labmanual/01CELLS.htm
http://www.bioweb.uncc.edu/1110Lab/notes/notes1/labpics/Elodea%20Leaf40c.jpghttp://www.bioweb.uncc.edu/1110Lab/notes/notes1/labpics/Elodea%20Leaf40c.jpg
Dissecting microscope • Focuses light • See surface of objects • Magnifies up to 40 times • Can view live organisms • Uses combination of lenses wappingersschools.org/.../visualvocab/page4.html
http://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/charophyceans/Chara_d2_LApond2e_3.15x.jpghttp://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/charophyceans/Chara_d2_LApond2e_3.15x.jpg
Electron microscope Two types: • Transmission electron microscope (TEM) • Scanning electron microscope (SEM)
Transmission electron microscope • Focuses electrons • Sees through objects • Magnifies up to 100,000 times • Can view only dead or inanimate objects www.phy.cuhk.edu.hk/.../CM120/CM120.html
Transmission electron microscope Mammalian mitochondrion biology.unm.edu/.../Summaries/Cell.html
Scanning electron microscope • Uses electrons • View surface of objects • Magnifies up to 10,000 times • Views only dead or inanimate objects http://gsc.nrcan.gc.ca/ebeam/sem_e.php
Scanning electron microscope Bee eye www.rdg.ac.uk/cfam/facilities/sem.htm
Viruses budding from a cell Transmission electron microscope Scanning electron miscroscope www. aecom.yu.edu/aif/gallery/sem/sem.htm
JEOL microscope • Uses finely-focused electrons • surface • Magnifies up to at least 2,000,000 times • Cannot view live organisms