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Breast Cancer ECM Visualization using Multiphoton Imaging

Learn how to visualize breast cancer extracellular matrix using advanced multiphoton imaging techniques. Follow the detailed protocol for staining and imaging samples to study different tissue structures and cancer types.

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Breast Cancer ECM Visualization using Multiphoton Imaging

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  1. Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research

  2. H&E and SHG • Protocol • Thaw & fix sample • Section sample to 200 microns on a vibratome • Image with SHG • Paraffin embed tissue wafer • Section and stain for H&E • Slide scan • Green = SHG signal (Collagen Type I)

  3. Normal Associated Tissue H&E SHG 11.6 mm

  4. Invasive Ductal Carcinoma SHG H&E

  5. Fibroadenoma H&E SHG

  6. Mucinous Carcinoma 7.5 mm H&E SHG

  7. Intrinsic Fluorescence • Green = SHG • Red = Autofluorescence from FAD or Collagen type III

  8. FibroAdenoma

  9. Extrinsic Fluorescence • Green = SHG (Collagen type I) • Red = E-Cadherin stained cells & Autofluorescence from Collagen type III

  10. Terminal Ductal Lobular Unit (Red) Surrounded by Collagen (Green)

  11. Duct in Cross Section, Surrounded by IDC cells IDC Duct

  12. IDC Cells Near a Vessel with ECM IDC Cells Vessel

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