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Lamellar Characterization of Bone

Lamellar Characterization of Bone. Presented by: Melanie Patel Advisors: Dr. Surya Kalidindi Dr. Haviva Goldman. Definition. Bone is a stiff skeletal material made principally of the fibrous protein collagen, impregnated with a mineral closely resembling calcium phosphate.

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Lamellar Characterization of Bone

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  1. Lamellar Characterization of Bone Presented by: Melanie Patel Advisors: Dr. Surya Kalidindi Dr. Haviva Goldman

  2. Definition • Bone is a stiff skeletal material made principally of the fibrous protein collagen, impregnated with a mineral closely resembling calcium phosphate. • A composite material consisting of fibrous protein, collagen, stiffened by extremely dense filling and surrounding of calcium phosphate crystals. • The mineralized collagen fibrils are considered the basic building block of the bone.

  3. Seven Hierarchical Levels

  4. Collagen • Basic building block of collagenous framework is a triple helical molecule • 3 amino acids: glycine, proline, hydroxyproline. • The structure follows the sequence of Glycine-X-Y where X is proline and Y can be either proline or hydroxyproline. • Hodge and Petruska Model

  5. Bone Structure

  6. Purpose • Characterize Lamellar Structure • Quantitative • Orientation • Chemical Make-up • Size (Thickness) • Use information to predict Macroscale Properties.

  7. Lamellae • The individual lamellae of bone consist of a fibril matrix with collagen fibers interweaving each other • Thin Lamellae • dense, fiber-rich, lack substance • 1-3 μm • Thick Lamellae • Collagen-poor layer, more substance • 2-4 μm

  8. Theories • Plywood Structure- orthogonally alternating layers • Fiber orientation at the Boundary- Ascenzi and Benvenuti, fiber directions do not change abruptly • 3-D Organization- Marotti • Twisted Plywood- gives rise to arc pattern in osteons • Rotated Plywood

  9. Microscopy • Polarized Light • SEM • TEM

  10. Characterization • Nanoindentation • AFM • EDAX??

  11. Experimentation thus far • Femoral bone sample obtained • Samples mounted in PMMA solidified • Cut, grind, polish • Light Microscopy • Nanoindentation, AFM • EDAX Experimentation plans

  12. Sources • Curry, John D. “Bones: Structure and Mechanics”. 2002. • Weiner S. and Wagner H.D. “The Material Bone: Structure-Mechanical Function Relations”. Annu. Rev. Mater. Sci. (28). 1998: 271-298. • Weiner, Stephen and Traub, Wolfie. “Bone Structure: from angstroms to microns” Faseb Journal. (6) 1992: 879-885. • Weiner S. et al. “Rotated Plywood Structure of Primary Lamellar Bone in the Rat: Orientations of the Collagen Fibril Arrays” Bone. (20) 1997: 509-514. • Marotti, Gastone and Muglia, Maria Antonietta. “A scanning electron microscope study of human bone lamellae. Proposal for a new model of collagen lamellar organization.” Arch. Ital. Anat. Embroil. (93) 1988: 163-175. • Giraud-Guille, M.M. “Twisted Plywood Architecture of Collagen Fibrils in Human Compact Bone Osteons” Calcif Tissue Int. (42) 1988: 167-180. • Ziv, V. et al. “Microstructure-Microhardness Relations in Parellel-Fibered and Lamellar Bone” Bone. (18) 1996: 417-428. • Ascenzi A. and Benvenuti A. “Orientation of Collagen Fibers at the Boundary Between Two Successive Osteonic Lamellae and its Mechanical Interpretation” J. Biomechanics. (19) 1986: 455-463. • Bromage, Timothy G. et al. “Circularly Polarized Light Standard for Investigations of Collagen Fiber Orientation in Bone” The Anatomical Record. (274B) 2003: 157-168. • Rubin, Matthew et al. “TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone” Bone. (33) 2003: 270-282.

  13. Questions ?

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