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fig19-16

electron transport chain. fig19-16. fig19-17. proton-motive force. 23.6 kJ/mole H +. the coupling question…. chemiosmotic hypothesis!. the coupling question…. chemiosmotic hypothesis!. fig19-19. oxidative phosphorylation experiment. measure this. add this. fig19-20. ADP + P i.

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fig19-16

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  1. electron transport chain fig19-16

  2. fig19-17

  3. proton-motive force 23.6 kJ/mole H+

  4. the coupling question… chemiosmotic hypothesis!

  5. the coupling question… chemiosmotic hypothesis!

  6. fig19-19

  7. oxidative phosphorylation experiment measure this add this

  8. fig19-20

  9. ADP + Pi ATP oxidative phosphorylation experiment measure this add this

  10. fig19-20

  11. oxidation and phosphorylation are coupled!!! fig19-20

  12. oxidation and phosphorylation are coupled!!! fig19-20

  13. ADP + Pi ATP oxidative phosphorylation experiment measure this add this

  14. ADP + Pi ATP oxidative phosphorylation experiment remove this this stops

  15. ADP + Pi ATP oxidative phosphorylation experiment block ATPase this stops

  16. weak acid uncouplers high H+ low H+

  17. Weak acid uncouplers fig19-21

  18. Chemical uncouplers fig19-21

  19. Chemical uncouplers fig19-21

  20. Chemical uncoupler experiment fig19-20

  21. Proton gradient experiment fig19-22

  22. Ask… or txt: 858 914 4945

  23. ATP synthase: the F1 subunit

  24. ATP and ADP are in rapid equilibrium in the F1 ATPase fig19-23

  25. Energetics of ATP and ADP in F1 ATPase… fig19-24

  26. The “binding-change” model for ATP synthase fig19-25

  27. The “binding-change” model for ATP synthase fig19-25

  28. The “binding-change” model for ATP synthase fig19-26

  29. The F1 complex of the ATPase… fig19-25

  30. The F0F1 complex fig19-25

  31. The F0F1 complex soluble membrane fig19-25

  32. The F0F1 complex of the ATPase… fig19-25

  33. The F0F1 complex of the ATPase… fig19-25

  34. A direct test of the rotary mechanism fig19-25 Noji et al. 1997

  35. A direct test of the rotary mechanism

  36. Snapshots from the direct test of rotary mechanism… fig19-27

  37. The movie version of the book

  38. Ask… or txt: 858 914 4945

  39. Bacterial generation of H+ gradient by respiratory chain an ancient mode of ATP production

  40. “There’s more than one way to shrink a gradient…” fig19-39

  41. Inner membrane translocases (or transporters) fig19-28

  42. Glycerol 3-P shuttle fig19-30

  43. Malate-aspartate shuttle eeeeek! fig19-29

  44. Malate-aspartate shuttle NADH NAD+

  45. Malate-aspartate shuttle X

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