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Advancements in Seeding for Crystal Formation: Techniques and Applications

Explore examples of new crystal structures obtained through critical seeding methods. Learn why seeding is essential, how to seed effectively, and what materials to use. Discover optimization techniques, microgravity applications, and strategies like streak and serial seeding. Understand the principles of seeding in the context of phase diagrams and crystallization. Delve into the nuances of seeding from different sources like oil, best crystals, or solid phases. Gain insights into enhancing crystallization success and protein-ligand interactions through seeding. This guide provides practical tips for improving crystal formation outcomes using seeding techniques.

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Advancements in Seeding for Crystal Formation: Techniques and Applications

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  1. Seeding Terese Bergfors Dept of Cell and Molecular Biology Uppsala University, Uppsala Sweden terese@alpha2.bmc.uu.se Examples of two new structures from our laboratory where seeding was critical to obtaining the crystals

  2. Seeding • Why seed? • How? • What to use as a seed?

  3. Some methods for optimization microgravity seeding

  4. Seeding and the phase diagram Luft and De Titta, Acta Cryst. (1999) D55, 988-993

  5. Serial seeding 20 mg/ml 10 mg/ml 10 mg/ml

  6. What to seed from? oil Take your best crystal or any solid phase

  7. Streak seeding whisker Touch crystal Draw streak line Enrico Stura in “Crystallization of Nucleic Acids and Proteins” Ed. Ducruix and Giege, 1992 Oxford University Press p. 108

  8. Does seeding solve everything? before after

  9. After Seeding, Ni Does seeding solve everything? After seeding, Ni, and ligand

  10. Summary of Tips • Lower concentration • Use any solid phase • Serial seed • Use sitting drops

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