1 / 17

Impact of Heavy Metal-Induced Oxidative Stress on Energy Metabolism in Drosophila melanogaster

This study examines the effect of heavy metals on the energy metabolism and oxidative stress tolerance in Drosophila melanogaster. The results show that heavy metal exposure leads to slowed growth and altered macromolecular accumulation, highlighting the role of bioenergetics in regulating oxidative stress tolerance. The study also suggests a positive correlation between energy reserves and stress tolerance, with females exhibiting greater tolerance than males.

waynewinter
Download Presentation

Impact of Heavy Metal-Induced Oxidative Stress on Energy Metabolism in Drosophila melanogaster

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Affect of heavy metal induced oxidative stress tolerance on energy metabolism in Drosophila melanogaster Geetanjali Sageena Evolutionary Biology Laboratory Department of Zoology University of Delhi New Delhi-110007

  2. Background • Industrialization and urbanization has resulted in environmental pollution. • Various Heavy metals are adding to environmental load due to higher rate of persistence and accumulation. • Results in oxidative stress. Persistent imbalance between antioxidants and pro-oxidants causing irreversible cellular damage. • Adaptive physiological mechanisms aid in increased survival which plays an important role in evolution and adaptation of living organisms.

  3. 22-24 h ~ 110 h ~ 24 h ~ 48 h ~ 24 h www.anatomy.unimelb.edu.au/.../life_cycle.jpg

  4. Fly maintenance Pre Adult Duration 9½ days Lifespan 30 Days Pre Adult Duration 7 days Lifespan 45 Days

  5. Control • Resource Rich • Short Lifespan • Selected • Resource Deficient • Long Lifespan

  6. Adult flies are housed in Plexiglas cages (25 x 20 x 15 cm3) Banana-jaggery food is provided in petridish and changed every alternate day. Populations kept at standard laboratory conditions (SLC).

  7. Aims and Objectives • To check the effect/affect of two chemical stressors (Heavy Metals) namely FeSO4 and CdCl2 on L1 to adult development. • Estimation of major energy reserves in both stressed and non stressed condition

  8. Methodology • Two types of fly populations • Heavy Metal Conc. CONTROL (JBs) SELECTED (FLJs) YEAST SM 1 SM + 13 mM FeSO4 Sync Eggs 2 SM + 5µM CdCl2 3 4

  9. L1 to adult development time. • Estimation of major energy reserves i.e. Lipids, Proteins and Carbohydrates by Ether, BCA and Anthronemethod respectively • Estimation of Energy accumulation per unit time. (Platt and Irwin 1973)

  10. RESULTS developmental Time

  11. Lipid content

  12. PROTEIN content

  13. carbohydrates content

  14. energy accumulation/ time Treatment F2,4 = 16.107, p = 0.01

  15. CONCLUSIONS • Larval growth slowed when reared on heavy metal supplemented diet. • Increased development time did not result in the increased macromolecular accumulation among the flies.Slowing down of the metabolic processes, or a greater utilization of energy reserves during metamorphosis or both. • Bioenergetics play a crucial role in regulation of Oxidative stress. • Positive relation between energy reserve and stress tolerance. Whereas stress tolerance and lifespan are not tightly correlated. • Females more tolerant than males.

  16. Acknowledgements My Supervisor Dr. Mallikarjun Shakarad Funding agency CSIR &DU Experimental Work: Mr. Rakesh Roshan Ms Shreya Choudhary Ms Nalini Mishra Laboratory Assistance: Sahil Malhotra

  17. THANK YOU

More Related